| 產品編號 | bs-0271R |
| 英文名稱 | HCMV pp65 Rabbit pAb |
| 中文名稱 | 巨細胞病毒PP65/CMV低基質磷脂蛋白抗體 |
| 別 名 | PP65_HCMVM; PP65_HCMVA; Cytomegalovirus pp65; 65 kDa lower matrix phosphoprotein; 65 kDa matrix phosphoprotein; 65 kDa phosphoprotein; CMV 65 kDa lower matrix phosphoprotein; CMV pp65; Cytomegalovirus 65 kDa lower matrix phosphoprotein; Cytomegalovirus pp65; HHV 5; PP65; Tegument protein pp65; Tegument protein UL83; UL83; HCMV PP65. |
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Specific References (5) | bs-0271R has been referenced in 5 publications.
[IF=20.693] Hanlin He. et al. ADAM9: A regulator between HCMV infection and function of smooth muscle cells. J MED VIROL. 2022 Nov;: IF ; Human.
[IF=4.936] Ma M et al. LncRNA XIST mediates bovine mammary epithelial cell inflammatory response via NF‐κB/NLRP3 inflammasome pathway.(2018) Cell Prolif. WB ; Bovine.
[IF=4.936] Wang et al. Octreotide Protects the Mouse Retina against Ischemic Reperfusion Injury through Regulation of Antioxidation and Activation of NF-κB. (2015) Oxid.Med.Cell.Longev. 2015:970156 WB ; Mouse.
[IF=2.559] Dai M et al.Pravastatin sodium attenuated TREM-1-mediated inflammation in human peripheral blood mononuclear cells.(2019)Biochem Biophys Res Commun. Jan 1;508(1):225-229.Commun. WB ; Human.
[IF=0] Yin M et al. Detection of Human Cytomegalovirus in Patients with Epithelial Ovarian Cancer and its Impacts on Survival. 04 February 2020, PREPRINT (Version 1) available at Research Square. IHC-P ; Human.
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| 研究領域 | 細胞生物 免疫學 細菌及病毒 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human (predicted: HCMVPP65) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 65 kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from HCMV pp65: 251-350/561 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Cytomegalovirus is a member of the herpes virus group, which includes herpes simplex virus types 1 and 2, varicella zoster virus (which causes chicken pox), and Epstein Barr virus (which causes infectious mononucleosis). These viruses share a characteristic ability to remain dormant within the body over a long period. CMV viral genes are co-ordinately expressed in groups at various times after infection. Early viral proteins are expressed in the nucleus of infected cells within 3 to 24 hours of infection prior to the commencement of viral DNA replication. This is followed by expression of the early intermediate genes, which encode enzymes required for viral DNA replication. After 48 to 72 hours, a number of late viral antigens may be demonstrated in the nuclei and cytoplasm of infected cells. pp65 is a 65kD phosphorylated glycoprotein and is the most abundant of the late antigens. Function: Counteracts the host antiviral immune response by preventing IRF3 to enter the nucleus once activated and phosphorylated. Participates also in the transactivation of viral major imediate-early genes by recruiting host IFI16 to their promoters. Subunit: Interacts with host NCL/nucleolin. Interacts with host IFI16. Subcellular Location: Virion tegument (Potential). Host nucleus. Host cytoplasm. Note=As part of the incoming virion, pp65 is targeted to the nucleus immediately after infection. The newly synthesized pp65 is observed in the nucleus until some time after 48 hours postinfection. Thereafter, pp65 is probably exported and accumulates in the cytoplasm. Also found in dense bodies. Post-translational modifications: Phosphorylation may play a role in the localization of the protein. Similarity: Belongs to the herpesviridae pp65 family. Database links: Entrez Gene: 3077579 Human herpesvirus 5 SwissProt: Q6SW59 Human herpesvirus 5 細胞巨化病毒是一種皰疹病毒, 易引起先天性畸形，且患者在接受大量輸血和免疫抑制力治療時它是引起并發癥的主要生物因子。 巨細胞病毒是一種感染肺、腎、腸和其他器官的條件致病菌。該抗體識別分子量為52、65、72以及86kDa的立即早期非結構抗原。該抗原在感染2小時后即可被檢測到。而感染96小時達到峰值，并持續整個CMV周期。 初期感染此病毒的孕婦中近一半會將此病傳播給她的胎兒，被傳染的胎兒長大后易智力低下，失明或耳聾。 |
| 產品圖片 |
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cytomegalovirus pp65 Polyclonal Antibody, Unconjugated(bs-0271R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
