| 產品編號 | bs-0505R |
| 英文名稱 | Sodium hydrogen exchanger 1 Rabbit pAb |
| 中文名稱 | 鈉氫通道蛋白抗體 |
| 別 名 | amiloride-sensitive; APNH; APNH1; FLJ42224; Na Li countertransporter; Na(+)/H(+) antiporter; Na(+)/H(+) exchanger 1; Na+H+antiporter amiloride-sensitive; Na+H+antiporter; Na+H+exchanger 1; NHE-1; NHE1; OTTHUMP00000004468; SL9A1_HUMAN; SLC9A1; Sodium hydrogen exchanger 1; Sodium/hydrogen exchanger 1; solute carrier family 9; Solute carrier family 9 member 1; Solute carrier family 9 sodium hydrogen exchanger isoform 1 antiporter Na+H+amiloride sensitive; Solute carrier family 9 subfamily A(NHE1 cation proton antiporter 1) member 1; Solute carrier family 9 subfamily A member 1. |
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Specific References (2) | bs-0505R has been referenced in 2 publications.
[IF=7.675] Ya-Ling Yin. et al. Citronellal Attenuates Oxidative Stress–Induced Mitochondrial Damage through TRPM2/NHE1 Pathway and Effectively Inhibits Endothelial Dysfunction in Type 2 Diabetes Mellitus. ANTIOXIDANTS-BASEL. 2022 Nov;11(11):2241 IHC, IF ; Rat, Human.
[IF=5.714] Yaling Yin. et al. PAE ameliorates doxorubicin-induced cardiotoxicity via suppressing NHE1 phosphorylation and stimulating PI3K/AKT phosphorylation. INT IMMUNOPHARMACOL. 2022 Dec;113:109274 IHC ; Rat.
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| 研究領域 | 心血管 細胞生物 信號轉導 通道蛋白 轉運蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 91 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NHE1: 470-550/815 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a Na+/H+ antiporter that is a member of the solute carrier family 9. The encoded protein is a plasma membrane transporter that is expressed in the kidney and intestine. This protein plays a central role in regulating pH homeostasis, cell migration and cell volume. This protein may also be involved in tumor growth. [provided by RefSeq, Sep 2011] Function: Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. Subunit: Oligomer. Interacts with calmodulin and TESC. Interacts (via the juxtamembrane region of the cytoplasmic C-terminus domain) with CHP1; the interaction occurs at the plasma membrane in a calcium-dependent manner. Interacts with CHP2; the interaction occurs in a calcium-dependent manner. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Kidney and intestine. Post-translational modifications: O-glycosylated. Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is reduced by CHP1. Similarity: Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. SWISS: P19634 Gene ID: 6548 Database links: Entrez Gene: 6548 Human Entrez Gene: 20544 Mouse Omim: 107310 Human SwissProt: P19634 Human SwissProt: Q61165 Mouse Unigene: 469116 Human Unigene: 4312 Mouse Unigene: 5025 Rat 通道蛋白（Channel Protein） 鈉-氫交換蛋白（NHE： Na+/H+ Exchanger）是維持細胞內環境的主要細胞膜蛋白質之一，它的一個主要功能是調節細胞內pH值，使之保持在生理范圍之內。細胞內pH值的變化，直接或間接的影響細胞的生長分裂、增殖和分化，也影響細胞的死亡。 鈉-氫交換蛋白質的活性還影響細胞的移動和腫瘤細胞的轉移。 目前主要方向是：研究消化道腫瘤細胞轉移。 |
| 產品圖片 |
Sample:
Brain(Rat) lysate at 30ug;
Liver(Rat) lysate at 30ug;
Primary: Anti-NHE1 (bs-0505R) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution;
Predicted band size : 91kD
Observed band size : 91kD
We are unsure as to the identity of these extra bands
Sample:
molt-4(human)cell Lysate at 40 ug
Primary: Anti- Sodium hydrogen exchanger 1 (bs-0505R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 91kD
Observed band size: 91 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Sodium hydrogen exchanger 1) Polyclonal Antibody, Unconjugated (bs-0505R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
