| 產品編號 | bs-1500R |
| 英文名稱 | Cathepsin B Rabbit pAb |
| 中文名稱 | 組織蛋白酶B抗體 |
| 別 名 | Amyloid precursor protein secretase; APP secretase; APPS; Cathepsin B; CB; Cathepsin B1; CathepsinB; CPSB; CTSB; Cysteine protease; Preprocathepsin B; Cathepsin B heavy chain; Cathepsin B light chain; CATB_HUMAN. |
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Specific References (4) | bs-1500R has been referenced in 4 publications.
[IF=10.19] Xingliang Dai. et al. Crosstalk between microglia and neural stem cells influences the relapse of glioblastoma in GBM immunological microenvironment. CLIN IMMUNOL. 2023 Jun;251:109333 IHC ; Human.
[IF=4.372] Lin Tianji. et al. Methylmercury induces lysosomal membrane permeabilization through JNK-activated Bax lysosomal translocation in neuronal cells. Toxicol Lett. 2022 Jan;: IF ; Human.
[IF=2.438] Liru Li. et al. Rapamycin Pretreatment Alleviates Cerebral Ischemia/Reperfusion Injury in Dose-Response Manner Through Inhibition of the Autophagy and NFκB Pathways in Rats:. Dose-Response. 2020;18(3): WB,IF ; Rat.
[IF=0.18] Liu, B., et al. "Autophagy activation aggravates neuronal injury in the hippocampus of vascular dementia rats." Neural Regeneration Research 9.13 (2014): 1288. IHC-P ; Rat.
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| 研究領域 | 腫瘤 免疫學 合成與降解 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 23/28/37 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cathepsin B heavy chain: 251-339/339 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer disease, the most common cause of dementia. Overexpression of the encoded protein, which is a member of the peptidase C1 family, has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] Function: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. Subunit: Dimer of a heavy chain and a light chain cross-linked by a disulfide bond. Interacts with SRPX2. Subcellular Location: Lysosome. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Similarity: Belongs to the peptidase C1 family. SWISS: P07858 Gene ID: 1508 Database links: Entrez Gene: 1508 Human Entrez Gene: 13030 Mouse Omim: 116810 Human SwissProt: P07858 Human SwissProt: P10605 Mouse Unigene: 520898 Human Unigene: 236553 Mouse Unigene: 472420 Mouse Unigene: 100909 Rat 合成與降解（Synthesis and Degradation） 組織蛋白酶B(CB) 參與腫瘤的侵襲轉移過程。可促進腫瘤血管的增生，增強腫瘤細胞的運動能力.組織蛋白酶B轉錄以及表達水平的高低與腫瘤預后呈正相關。 cathepsin B屬于半胱氨酸蛋白酶,與人體的多種生理、病理過程密切相關.組織蛋白酶B (cathepsin B,CB)主要存在于溶酶體中, 發揮其生理狀態下的蛋白水解作用.但在某些特定條件下, CB在胞外間質中有時也可以檢測到. 目前，有學者將CB用于阿爾茨海默病（AD）方面的研究。 |
| 產品圖片 |
Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-Cathepsin B (bs-1500R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46/28 kD
Observed band size: 30 kD
Tissue/cell: Human esophageal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cathepsin B Polyclonal Antibody, Unconjugated(bs-1500R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: RSC96(blue). Primary Antibody:Rabbit Anti- Cathepsin B antibody(bs-1500R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . Antibody (bs-1500R, 5μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-1500R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
