| 產(chǎn)品編號(hào) | bsm-1621M |
| 英文名稱 | AFP(A2) Mouse mAb |
| 中文名稱 | 甲胎蛋白單克隆抗體（包被） |
| 別 名 | alpha Fetoprotein; alpha-fetoprotein; alpha 1 fetoprotein; alpha-1-fetoprotein; alpha-fetoglobulin; alpha fetoglobulin; alpha Fetoprotein; alpha fetoprotein precursor; FETA; HPAFP; FETA_HUMAN. |
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Specific References (12) | bsm-1621M has been referenced in 12 publications.
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| 研究領(lǐng)域 | 腫瘤 |
| 抗體來(lái)源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號(hào) | A2 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 65 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | AFP protein: full length |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M PBS (pH7.4) with 0.02% Proclin300. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries. Subunit: Dimeric and trimeric forms have been found in addition to the monomeric form. Subcellular Location: Secreted. Tissue Specificity: Plasma. Synthesized by the fetal liver and yolk sac. Similarity: Belongs to the ALB/AFP/VDB family. Contains 3 albumin domains. Database links: Entrez Gene: 174 Human Omim: 104150 Human SwissProt: P02771 Human Unigene: 518808 Human |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with AFP(A2) monoclonal antibody, unconjugated (bsm-1621M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AFP(A2) Monoclonal Antibody, Unconjugated(bsm-1621M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
(Negative control)Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AFP(A2) Monoclonal Antibody, Unconjugated(bsm-1621M) at 1:500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
(Negative control)Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AFP(A2) Monoclonal Antibody, Unconjugated(bsm-1621M) at 1:500 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Measured by its binding ability in a indirect ELISA. Immobilized Human AFP protein, His Tag (Cat. bs-43140P) at 2 μg/mL (100 μL/well) can bind ? Mouse Anti-Human AFP Antibody, the EC50 is 24.94 ng/mL.
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