| 產品編號 | bs-1825R |
| 英文名稱 | Inhibin beta B Rabbit pAb |
| 中文名稱 | 抑制素βB/Inhibin β B抗體 |
| 別 名 | MGC157939; Activin A; Activin AB alpha polypeptide; Activin AB; Activin AB beta polypeptide; Activin B; Activin beta A chain; Activin beta B chain; ActivinA; ActivinAB; ActivinB; EDF; Erythroid differentiation protein; follicle stimulating hormone releasing protein; FRP; FSH releasing factor; FSH releasing protein; INHBA; INHBB; Inhibin; Inhibin beta 1; Inhibin beta 2; Inhibin beta A; Inhibin beta A chain; Inhibin beta A subunit; Inhibin beta B; Inhibin beta B chain; Inhibin beta B subunit; Inhibin βB. |
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Specific References (1) | bs-1825R has been referenced in 1 publications.
[IF=3.998] A. Ikeda. et al. Follistatin expressed in mechanically-damaged salivary glands of male mice induces proliferation of CD49f + cells. Sci Rep-Uk. 2020 Nov;10(1):1-14 IHC ; Mouse.
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| 研究領域 | 神經生物學 信號轉導 生長因子和激素 轉錄調節因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat (predicted: Pig,Sheep,Cow,Dog,GuineaPig) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 13/45 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Inhibin beta B: 251-350/407 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The inhibin beta B subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumour-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta B subunit forms a homodimer, activin B, and also joins with the beta A subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. [provided by RefSeq]. Subcellular Location: Secreted. Similarity: Belongs to the TGF-beta family. SWISS: P09529 Gene ID: 3625 Database links: Entrez Gene: 3625 Human Entrez Gene: 16324 Mouse Omim: 147390 Human SwissProt: P09529 Human SwissProt: Q04999 Mouse Unigene: 1735 Human Unigene: 3092 Mouse Unigene: 35074 Rat 抑制素是轉化生長因子TGFβ超級家族，主要由雌性動物卵巢顆粒細胞和雄性動物睪丸的Sertoli細胞分泌.研究表明,抑制素具有內分泌和旁分泌物的作用:既可通過復雜的反饋機理,調節體液FSH水平。 |
| 產品圖片 |
Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti- Inhibin beta B (bs-1825R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 50 kD
Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Breast tissue lysates
Lane 3: Mouse Placenta tissue lysates
Lane 4: Mouse Liver tissue lysates
Lane 5: Rat Testis tissue lysates
Lane 6: Rat Breast tissue lysates
Lane 7: Human A431 cell lysates
Lane 8: Human HepG2 cell lysates
Primary: Anti- Inhibin beta B (bs-1825R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13/45 kDa
Observed band size: 46 kDa
Sample:
A431(Human) Cell Lysate at 40 ug
Primary: Anti- Inhibin beta B (bs-1825R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (rat ovary tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Inhibin beta B) Polyclonal Antibody, Unconjugated (bs-1825R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
