| 產品編號 | bs-2109R |
| 英文名稱 | V5 tag Rabbit pAb |
| 中文名稱 | V5-tag標簽抗體 |
| 別 名 | V5-tag; GKPIPNPLLGLDST epitope tag; GKPIPNPLLGLDST tag; Protein Rev; Regulator of expression of viral proteins; rev; V5 epitope tag. |
| 產品類型 | 標簽抗體 |
| 研究領域 | 免疫學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Recombinant protein |
| 產品應用 | WB=1:10000-100000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated V5 tag |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids, although it has also been used with a shorter 9 amino acid sequence. 該抗體能特異性識別位于重組蛋白C端或N端的V5標簽。 |
| 產品圖片 |
Sample: Lane1: V5-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug Lane2: V5-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug Primary: Anti-V5 tag (bs-2109R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD
Sample:
Lane 1: V5-Tagged Fusion Protein Overexpression E.coli Lysate (bs- 41230P) at 10ug
Primary: Anti-V5 tag (bs-2109R) at 1/100000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 45 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
