97久久久精品综合88久久_亚洲国产精品一_久热热国产久热_97操操操_北条麻妃在线免费观看_精品国自产拍天天拍

掃碼關注公眾號           掃碼咨詢技術支持           掃碼咨詢技術服務
  
客服熱線:400-901-9800  客服QQ:4009019800  技術答疑  技術支持  質量反饋  關于我們  聯系我們
青青草18在线视频免费,国产日产欧产精品精品软件,日本aⅴ精品中文字幕
首頁 > 產品中心 > 一抗 > 產品信息
phospho-ATF2 (Thr69/71) Rabbit pAb (bs-3033R)  
訂購熱線:400-901-9800
訂購郵箱:sales@bioss.com.cn
訂購QQ:  400-901-9800
技術支持:techsupport@bioss.com.cn
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包裝/詢價

產品編號 bs-3033R
英文名稱 phospho-ATF2 (Thr69/71) Rabbit pAb
中文名稱 磷酸化活化復制因子2抗體
別    名 ATF2(phospho T69+T71); ATF2(phospho Thr69+Thr71); p-ATF2(T69+T71); p-ATF2(Thr69+Thr71); CREB 2; HB 16; Activating Transcription Factor 2; ATF 2; Atf-2; ATF2 protein; cAMP Response Element Binding Protein 2; cAMP response element binding protein CRE BP1; cAMP-dependent transcription factor ATF-2; cAMP-responsive element-binding protein 2; CRE BP1; CRE-BP; CREB 2; CREB2; CREBP1; Cyclic AMP dependent transcription factor ATF 2; Cyclic AMP-responsive; ATF2_HUMAN.  
產品類型 磷酸化抗體 
研究領域 免疫學  染色質和核信號  轉錄調節因子  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Mouse,Rat (predicted: Human,Rabbit,Cow,Horse)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,Flow-Cyt=2ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 45 kDa
檢測分子量
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human ATF2 around the phosphorylation site of Thr69/71: DQ(p-T)P(p-T)PT 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 ATF2 is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.

Subunit:
Binds DNA as a dimer and can form a homodimer in the absence of DNA. Can form a heterodimer with JUN. Interacts with SMAD3 and SMAD4. Binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity.

Subcellular Location:
Nucleus.

Tissue Specificity:
Abundant expression seen in the brain.

Post-translational modifications:
Phosphorylation of Thr-69 by MAPK14 and MAPK11, and at Thr-71 by MAPK1/ERK2, MAPK3/ERK1, MAPK11, MAPK12 and MAPK14 in response to external stimulus like insulin causes increased transcriptional activity. Phosphorylated by PLK3 following hyperosmotic stress. Also phosphorylated and activated by JNK and CaMK4.

Similarity:
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger.

SWISS:
P15336

Gene ID:
1386

Database links:

Entrez Gene: 1386 Human

Entrez Gene: 100047997 Mouse

Entrez Gene: 11909 Mouse

Entrez Gene: 81647 Rat

Omim: 123811 Human

SwissProt: P15336 Human

SwissProt: P16951 Mouse

SwissProt: Q00969 Rat

Unigene: 592510 Human

Unigene: 209903 Mouse

Unigene: 9825 Rat



轉錄調節因子(Transcriptin Regulators)
產品圖片
Paraformaldehyde-fixed, paraffin embedded (human pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (phospho-ATF2 (Thr69/71)) Polyclonal Antibody, Unconjugated (bs-3033R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-ATF2 (Thr69)) Polyclonal Antibody, Unconjugated (bs-3033R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-ATF2 (Thr69)) Polyclonal Antibody, Unconjugated (bs-3033R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control:Mouse spleen. Primary Antibody (green line): Rabbit Anti-THEMIS antibody (bs-3033R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
版權所有 2004-2026 www.kastlife.cn 北京博奧森生物技術有限公司
通過國際質量管理體系ISO 9001:2015 GB/T 19001-2016    證書編號: 00124Q34771R2M/1100
通過國際醫療器械-質量管理體系ISO 13485:2016 GB/T 42061-2022    證書編號: CQC24QY10047R0M/1100
京ICP備05066980號-1         京公網安備110107000727號
主站蜘蛛池模板: 久久高清中文字幕 | 免费又黄又爽又猛的毛片 | 牛鞭擦进少妇的下身 | 无码一区二区三区在线观看 | 内射白浆一区二区在线观看 | 亚洲日本精品一区 | 成人毛片100部 | 精品无码国产自产在线观看水浒传 | 亚洲欧美日韩国产自偷 | 日日日日日日bbbbb视频 | 台湾av一区二区三区 | 久久久久久国产精品免费播放 | 亚洲欭美日韩颜射在线二 | 亚洲一区二区精彩视频 | 欧美日本久久久 | 亚洲一区二区在线免费视频 | 亚洲国产精品视频一区二区 | 欧美一区二区三区蜜桃 | 亚洲欧美闷骚少妇影院 | 丁香花在线观看免费观看 | 麻豆精品国产 | 亚洲综合成人婷婷五月在线观看 | 天天干夜夜爽 | 美女精品久久久 | 欧美精品videosex极品 | 视频免费观看的网站 | 国产精品久久久久久久婷婷 | 亚洲色图狠狠爱 | 啊灬啊灬啊灬快灬高潮了听书 | 国产精品一区二区三区视频网站 | 加勒比色老久久爱综合网 | 人人射人人 | 性夜影院爽黄A爽免费 | 久久精品欧洲AV无码四区 | 吃奶呻吟打开双腿做受视频 | 992tv国产精品福利在线 | 久草在线免费在线观看 | 国产99久久亚洲综合精品西瓜tv | 隔壁人妻被水电工征服 | 天堂AV无码大芭蕉伊人AV孕妇 | 日韩在线播放第一页 |