| 產品編號 | bs-3259R |
| 英文名稱 | Phospho-MAP2 (Ser136) Rabbit pAb |
| 中文名稱 | 磷酸化微管相關蛋白2抗體 |
| 別 名 | MAP2(phospho Ser136); MAP2(phospho S136); p-MAP2(phospho S136); MAP2(phospho S136); MAP2(phospho-Ser136); p-MAP2(phospho-Ser136); DKFZp686I2148; Dendrite specific MAP; DKFZp686I2148; MAP 2; MAP-2; MAP2; MAP2_HUMAN; MAP2A; MAP2B; MAP2C; Microtubule associated protein 2; Microtubule-associated protein 2; Mtap 2. |
| 產品類型 | 磷酸化抗體 |
| 研究領域 | 免疫學 神經生物學 信號轉導 干細胞 細胞凋亡 細胞類型標志物 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Rat (predicted: Mouse,Rabbit,Pig,Cow,Dog) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=0.2μg /Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 70/201 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human MAP2 around the phosphorylation site of Ser136: PP(p-S)P |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton. Function: The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. Subcellular Location: Cytoplasm, cytoskeleton (Probable). Post-translational modifications: Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). MAP2A/c is phosphorylated. Isoform MAP2c is phosphorylated by FYN at Tyr-67. Similarity: Contains 3 Tau/MAP repeats. SWISS: P11137 Gene ID: 4133 Database links: Entrez Gene: 4133 Human Entrez Gene: 17756 Mouse Omim: 157130 Human SwissProt: P11137 Human SwissProt: P20357 Mouse Unigene: 368281 Human Unigene: 256966 Mouse Unigene: 10484 Rat 微管相關蛋白 2（MAP-2)是一種磷蛋白質，在正常腦組織中，主要存在于神經元的胞體、樹突和樹突棘，是腦內最豐富的蛋白之一。MAP-2在其調節(jié)微管的聚合作用和微管的穩(wěn)定性以及對神經元軸突和樹突的發(fā)生、延長、穩(wěn)定和突觸可塑性調節(jié)具有重要作用。 |
| 產品圖片 |
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-MAP2 (Ser136) Polyclonal Antibody, Unconjugated(bs-3259R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: RSC96 Cells(blue). Primary Antibody: Rabbit Anti-hospho-MAP2(Ser136)/FITC Conjugated antibody (bs-3259R-FITC), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
Blank control: SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-Phospho-MAP2 (Ser136) antibody (bs-3259R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
