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MARCKS Rabbit pAb (bs-2909R)  
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產品編號 bs-2909R
英文名稱 MARCKS Rabbit pAb
中文名稱 丙氨酸蛋白激酶C底物Marcks抗體
別    名 80 kDa protein; 80K L; 80K L protein; 80K-L protein; 80KL; 81 kDa protein, light chain; light chain; MACS; MARCKS; MARCS; MARCS_HUMAN; MGC52672; myristoylated alanine rich C kinase substrate; Myristoylated alanine rich protein kinase C substrate(MARCKS, 80K L); Myristoylated alanine rich protein kinase C substrate; Myristoylated alanine-rich C-kinase substrate; phosphomyristin; PKCSL; PRKCSL; protein kinase C substrate 80 kDa protein light chain; Protein kinase C substrate.  
研究領域 心血管  免疫學  信號轉導  激酶和磷酸酶  細胞骨架  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Rat (predicted: Human,Mouse,Rabbit,Pig,Cow,Dog,Horse)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 31 kDa
檢測分子量
細胞定位 細胞漿 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human MARCKS: 31-120/332 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008]

Function:
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

Subcellular Location:
Cytoplasm, cytoskeleton (Probable). Membrane; Lipid-anchor.

Post-translational modifications:
Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.

Similarity:
Belongs to the MARCKS family.

SWISS:
P29966

Gene ID:
4082

Database links:

Entrez Gene: 4082 Human

Entrez Gene: 17118 Mouse

Entrez Gene: 25603 Rat

Omim: 177061 Human

SwissProt: P29966 Human

SwissProt: P26645 Mouse

SwissProt: P30009 Rat

Unigene: 519909 Human

Unigene: 712721 Human

Unigene: 30059 Mouse

Unigene: 9560 Rat



MARCKS是一種分子量為32kDa的膜內蛋白,在組織中廣泛表達,但主要高表達在腦內的軸突、小直徑的樹突和樹突棘.
產品圖片
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MARCKS Polyclonal Antibody, Unconjugated(bs-2909R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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