| 產品編號 | bs-3313R |
| 英文名稱 | Phospho-Nur77 (Ser351) Rabbit pAb |
| 中文名稱 | 磷酸化孤兒核受體蛋白Nur77抗體 |
| 別 名 | NR4A1(phospho S351); NUR77(phospho S351); p-NUR77(phospho S351); GFRP 1; GFRP; GFRP1; Growth factor inducible nuclear protein N10; Growth Factor Inducible Nuclear Protein NP10; Growth Factor Response Protein 1; Hbr1; HMR; Hormone Receptor; MGC9485; N10; N10 nuclear protein; NAK 1; NAK1; NGFIB; NP 10; NP10; NR4A1; Nuclear hormone receptor NUR/77; Nuclear Hormone Receptor TR3; Nuclear Receptor Subfamily 4 Group A Member 1; NUR77; Orphan nuclear receptor HMR; Orphan nuclear receptor NR4A1; ST 59; ST59; Steroid receptor TR3; TR 3; TR3; TR3 orphan receptor; Early response protein NAK1. |
| 產品類型 | 磷酸化抗體 |
| 研究領域 | 腫瘤 心血管 細胞生物 免疫學 染色質和核信號 神經生物學 信號轉導 細胞凋亡 轉錄調節因子 糖尿病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Cow,Chicken,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 66 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human Nur77 around the phosphorylation site of Ser351: LP(p-S)KP |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011] Function: Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2. Subunit: Binds DNA as a monomer. Interacts with GADD45GIP1. Subcellular Location: Nucleus. Tissue Specificity: Fetal muscle and adult liver, brain and thyroid. Post-translational modifications: Phosphorylated at Ser-351 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli. Similarity: Belongs to the nuclear hormone receptor family. NR4 subfamily. Contains 1 nuclear receptor DNA-binding domain. SWISS: P22736 Gene ID: 3164 Database links: Entrez Gene: 3164 Human Entrez Gene: 15370 Mouse Omim: 139139 Human SwissProt: P22736 Human SwissProt: P12813 Mouse Unigene: 524430 Human Unigene: 119 Mouse Unigene: 10000 Rat 孤兒核受體蛋白Nur77是早期反應轉錄因子之一，多種刺激因子如缺氧可誘導其迅速表達，Nur77參與調控細胞增殖、存活、分化等過程。這類核受體是體內廣泛分布的配體依賴性的轉錄因子，通過調控基因協調表達，在機體的生長發育、新陳代謝，細胞分化及體內許多生長發育過程中發揮重要作用，其功能障礙將導致一系列疾病的發生，如；癌癥、糖尿病、肥胖、不孕不育等等。 |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Nur77(Ser351)) Polyclonal Antibody, Unconjugated (bs-3313R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
