產品編號 | bs-3548R |
英文名稱 | DTNB Rabbit pAb |
中文名稱 | 肌萎縮Dystrobrevin β蛋白抗體 |
別 名 | beta dystrobrevin; beta-dystrobrevin; DTN B; DTN-B; Dtnb; DTNB_HUMAN; Dystrobrevin beta; MGC17163; MGC57126. |
研究領域 | 細胞生物 免疫學 信號轉導 細胞骨架 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
克 隆 號 | |
交叉反應 | Rat (predicted: Human,Mouse,Rabbit,Cow,Chicken,Dog,Horse) |
產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 71 kDa |
檢測分子量 | |
細胞定位 | 細胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human DTNB: 261-370/627 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 |
Dystrobrevin beta is a component of the dystrophin-associated protein complex and interacts with dystrophin short form DP71, syntrophins SNTG1 and SNTG2. It localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. 4 isoforms produced by alternative splicing have been identified. Subunit: Interacts with dystrophin short form DP71 and syntrophins SNTG1 and SNTG2. Binds dystrobrevin binding protein 1 (By similarity). Subcellular Location: Cytoplasm. Tissue Specificity: Highly expressed in brain, kidney and pancreas. Similarity: Belongs to the dystrophin family. Dystrobrevin subfamily. Contains 1 ZZ-type zinc finger. SWISS: O60941 Gene ID: 1838 Database links: Entrez Gene: 1838 Human Entrez Gene: 13528 Mouse Omim: 602415 Human SwissProt: O60941 Human SwissProt: O70585 Mouse Unigene: 307720 Human Unigene: 286202 Mouse |
產品圖片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DTNB Polyclonal Antibody, Unconjugated(bs-3548R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |