| 產(chǎn)品編號 | bs-3633R |
| 英文名稱 | Synaptopodin Rabbit pAb |
| 中文名稱 | 突觸足蛋白抗體 |
| 別 名 | 9030217H17RIK; 9130229N11; 9330140I15Rik; AW046661; SYNAPTOPODIN; KIAA1029; SYNPO; SYNPO_HUMAN. |
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Specific References (2) | bs-3633R has been referenced in 2 publications.
[IF=3.73] Zhang, Xueming, et al. "Resolvin D1 Protects Podocytes in Adriamycin-Induced Nephropathy through Modulation of 14-3-3β Acetylation." PLOS ONE 8.6 (2013): e67471. IP ; Mouse.
[IF=0] Garovic, Vesna D., and Muthuvel Jayachandran. "DETECTING PODOCYTE INJURY IN DIABETIC NEPHROPATHY AND GLOMERULONEPHRITIS." U.S. Patent No. 20,170,003,299. 5 Jan. 2017. Human.
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| 研究領域 | 腫瘤 細胞生物 免疫學 神經(jīng)生物學 信號轉導 細胞凋亡 轉錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg /test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 102 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Synaptopodin: 601-700/903 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Synaptopodin is an actin-associated protein that may play a role in actin-based cell shape and motility. May be essential for the formation of spine apparatuses in spines of telencephalic neurons, involved in synaptic plasticity. The name synaptopodin derives from the protein's associations with postsynaptic densities and dendritic spines and with renal podocytes. Subunit: Interacts with BAIAP1. Interacts with actin (By similarity). Interacts (via PPxY motifs) with WWC1 (via WW domains). Subcellular Location: Cytoplasm, cytoskeleton (By similarity). Cell junction, tight junction (By similarity). Perikaryon (By similarity). Cell projection, dendritic spine (By similarity). Cell junction, synapse, postsynaptic cell membrane, postsynaptic density (By similarity). Cell junction, synapse (By similarity). Note=Localized at the tight junction of cells. In brain, localized to the postsynaptic densities and in the perikarya. Asssociated with dendritic spines of a subset of synapses (By similarity). Tissue Specificity: Expressed in brain, namely in the olfactory bulb, cerebral cortex, striatum, and hippocampus, but not in the cerebellum. Also expressed in the podocytes of kidney glomeruli. In the hippocampus, mainly expressed in the principal cell layer of the dentate gyrus and Ammon's horn. Similarity: Belongs to the synaptopodin family. SWISS: Q8N3V7 Gene ID: 11346 Database links: Entrez Gene: 11346 Human Entrez Gene: 104027 Mouse Omim: 608155 Human SwissProt: Q8N3V7 Human SwissProt: Q8CC35 Mouse Unigene: 435228 Human synaptopodin是一種與肌動蛋白微絲偶聯(lián)的蛋白，其富含脯氨酸,為線狀蛋白質，在動物體內(nèi)表達于腎小球的足細胞和后腦的突觸內(nèi)，synaptopodin被認為是足細胞特異的分化成熟標志物。 |
| 產(chǎn)品圖片 |
Sample: Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-Synaptopodin (bs-3633R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 102 kD
Observed band size: 76 kD
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-Synaptopodin (bs-3633R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 102 kD
Observed band size: 102 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Synaptopodin) Polyclonal Antibody, Unconjugated (bs-3633R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Synaptopodin) Polyclonal Antibody, Unconjugated (bs-3633R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control（black line）:SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-Synaptopodin antibody (bs-3633R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Positive control: RSC96
Isotype Control Antibody: Rabbit IgG;
Secondary Antibody: Goat anti-rabbit IgG-FITC;
Dilution: 1:200 in 1 X PBS containing 0.5% BSA
Primary Antibody catalog number: bs-3633R;
Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
