| 產品編號 | bs-3978R |
| 英文名稱 | ENO1 Rabbit pAb |
| 中文名稱 | MYC啟動子結合蛋白1抗體 |
| 別 名 | 2 phospho D glycerate hydro lyase; alpha enolase 1; alpha enolase; C myc promoter binding protein; EC 4.2.1.11; ENO1L1; Enolase 1(alpha); Enolase 1(alpha) like 1; Enolase 1; MBP 1; MBP1; MBPB1; MPB 1; MPB1; MYC promoter binding protein 1; NNE; Non neural enolase; phosphopyruvate hydratase; Plasminogen binding protein; PPH; Non Neuronal Enolase; ENOA_HUMAN. |
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Specific References (5) | bs-3978R has been referenced in 5 publications.
[IF=6.162] Zhang W et al. Cinnamaldehyde Enhances Antimelanoma Activity through Covalently Binding ENO1 and Exhibits a Promoting Effect with Dacarbazine. Cancers (Basel). 2020 Jan 29;12(2). pii: E311. IHF ; Mouse.
[IF=5.732] Sun Wei. et al. The effects of maternal anti-alpha-enolase antibody expression on the brain development in offspring. CLIN EXP IMMUNOL. 2022 Sep;: IF, WB ; Mouse.
[IF=3.943] Yihan Lu? et al. Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathy. Int Immunopharmacol. 2020 Aug;85:106563. IF ; Mouse.
[IF=3.647] WB ; human.
[IF=3.647] Weiyi Zhanget al. Cinnamaldehyde changes the dynamic balance of glucose metabolism by targeting ENO1. Life Sci
. 2020 Oct 1;258:118151. other ;
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| 研究領域 | 腫瘤 細胞生物 免疫學 染色質和核信號 信號轉導 轉錄調節因子 結合蛋白 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Cow) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 47 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ENO1: 51-150/434 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
ENO1 encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. Function: Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. Subunit: Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific. ENO1 interacts with PLG in the neuronal plasma membrane and promotes its activation. The C-terminal lysine is required for this binding. Subcellular Location: Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Isoform MBP-1: Nucleus. Tissue Specificity: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. Similarity: Belongs to the enolase family. SWISS: P13929 Gene ID: 2027 Database links: Entrez Gene: 2023 Human Omim: 172430 Human SwissProt: P06733 Human Unigene: 517145 Human |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with ENO1 polyclonal antibody, unconjugated (bs-3978R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Tissue/cell: Rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ENO1 Polyclonal Antibody, Unconjugated(bs-3978R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
