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BDH1 Rabbit pAb (bs-5041R)  
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產品編號 bs-5041R
英文名稱 BDH1 Rabbit pAb
中文名稱 3-羥丁酸脫氫酶抗體
別    名 3 hydroxybutyrate dehydrogenase(heart, mitochondrial); BDH; BDH-1; D beta hydroxybutyrate dehydrogenase, mitochondrial; 3-hydroxybutyrate dehydrogenase; D-beta-hydroxybutyrate dehydrogenase; mitochondrial; BDH_HUMAN .  
Specific References  (2)     |     bs-5041R has been referenced in 2 publications.
[IF=4.225]   
[IF=4.225] Li Huihui. et al. Trimetazidine Ameliorates Myocardial Metabolic Remodeling in Isoproterenol-Induced Rats Through Regulating Ketone Body Metabolism via Activating AMPK and PPAR α. Front Pharmacol. 2020 Aug;11:1255  WB ;  Rat.  
研究領域 腫瘤  細胞生物  免疫學  轉錄調節因子  轉運蛋白  線粒體  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human (predicted: Mouse,Rat,Rabbit,Horse)
產品應用 Flow-Cyt=3ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 33 kDa
檢測分子量
細胞定位 細胞漿 線粒體
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human BDH1: 241-343/343 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes a member of the short-chain dehydrogenase/reductase gene family. The encoded protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. The encoded protein catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008].

Subunit:
Homotetramer.

Subcellular Location:
Mitochondrion matrix.

Similarity:
Belongs to the short-chain dehydrogenases/reductases (SDR) family.

SWISS:
Q02338

Gene ID:
622

Database links:

Entrez Gene: 622 Human

Entrez Gene: 71911 Mouse

Omim: 603063 Human

SwissProt: Q02338 Human

SwissProt: Q80XN0 Mouse

Unigene: 274539 Human

Unigene: 293470 Mouse



產品圖片
Blank control: A431. Primary Antibody (green line): Rabbit Anti-BDH1 antibody (bs-5041R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min atroom temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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