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MYL9 Rabbit pAb (bs-2380R)  
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產品編號 bs-2380R
英文名稱 MYL9 Rabbit pAb
中文名稱 肌球蛋白調節多肽9(平滑肌亞型)抗體
別    名 MYL9_HUMAN; Myosin regulatory light polypeptide 9; 20 kDa myosin light chain; LC20; MLC-2C; Myosin RLC; Myosin regulatory light chain 2, smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1; MLC2; MRLC1; MYRL2; Myosin light chain.  
Specific References  (2)     |     bs-2380R has been referenced in 2 publications.
[IF=4.192] Jinwen Yuan. et al. Reparative Effects of Ethanol-Induced Intestinal Barrier Injury by Flavonoid Luteolin via MAPK/NF-κB/MLCK and Nrf2 Signaling Pathways. J Agr Food Chem. 2021;XXXX(XXX):XXX-XXX  WB ;  Human.  
[IF=0.786] Dong?QQ et al. SMYD3-associated pathway is involved in the anti-tumor effects of sulforaphane on gastric carcinoma cells. Food Sci Biotechnol. 2018 Mar 22;27(4):1165-1173.  WB ;  Human.  
研究領域 細胞生物  免疫學  信號轉導  細胞骨架  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Dog)
產品應用 IHC-P=1:100-500,IHC-F=1:200-1000,IF=1:200-1000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 20 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human MYL9: 2-100/172 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 MYL9 is the myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. MYL9 protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for MYL9 and the deduced 172-amino acid protein is highly conserved, with only 3 differences between the human and chicken proteins. Light chain phosphorylation causes the folded monomeric form of myosin to extend and assemble into filaments. This observation established the involvement of the MYL9 in conformational transitions of smooth muscle myosin.

Function:
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.

Subunit:
Myosin is a hexamer of 2 heavy chains and 4 light chains.

Tissue Specificity:
Smooth muscle tissues and in some, but not all, nonmuscle cells.

Post-translational modifications:
Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge (By similarity).

Similarity:
Contains 3 EF-hand domains.

SWISS:
P24844

Gene ID:
10398

Database links:

Entrez Gene: 10398 Human

Entrez Gene: 98932 Mouse

Entrez Gene: 296313 Rat

Omim: 609905 Human

SwissProt: P24844 Human

SwissProt: Q9CQ19 Mouse

SwissProt: Q64122 Rat

Unigene: 504687 Human

Unigene: 271770 Mouse

Unigene: 6870 Rat



產品圖片
Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYL9) Polyclonal Antibody, Unconjugated (bs-2380R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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