| 產品編號 | bs-2380R |
| 英文名稱 | MYL9 Rabbit pAb |
| 中文名稱 | 肌球蛋白調節多肽9(平滑肌亞型)抗體 |
| 別 名 | MYL9_HUMAN; Myosin regulatory light polypeptide 9; 20 kDa myosin light chain; LC20; MLC-2C; Myosin RLC; Myosin regulatory light chain 2, smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1; MLC2; MRLC1; MYRL2; Myosin light chain. |
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Specific References (2) | bs-2380R has been referenced in 2 publications.
[IF=4.192] Jinwen Yuan. et al. Reparative Effects of Ethanol-Induced Intestinal Barrier Injury by Flavonoid Luteolin via MAPK/NF-κB/MLCK and Nrf2 Signaling Pathways. J Agr Food Chem. 2021;XXXX(XXX):XXX-XXX WB ; Human.
[IF=0.786] Dong?QQ et al. SMYD3-associated pathway is involved in the anti-tumor effects of sulforaphane on gastric carcinoma cells. Food Sci Biotechnol. 2018 Mar 22;27(4):1165-1173. WB ; Human.
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| 研究領域 | 細胞生物 免疫學 信號轉導 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Dog) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:200-1000,IF=1:200-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 20 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MYL9: 2-100/172 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
MYL9 is the myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. MYL9 protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for MYL9 and the deduced 172-amino acid protein is highly conserved, with only 3 differences between the human and chicken proteins. Light chain phosphorylation causes the folded monomeric form of myosin to extend and assemble into filaments. This observation established the involvement of the MYL9 in conformational transitions of smooth muscle myosin. Function: Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. Subunit: Myosin is a hexamer of 2 heavy chains and 4 light chains. Tissue Specificity: Smooth muscle tissues and in some, but not all, nonmuscle cells. Post-translational modifications: Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge (By similarity). Similarity: Contains 3 EF-hand domains. SWISS: P24844 Gene ID: 10398 Database links: Entrez Gene: 10398 Human Entrez Gene: 98932 Mouse Omim: 609905 Human SwissProt: P24844 Human SwissProt: Q9CQ19 Mouse Unigene: 504687 Human Unigene: 271770 Mouse Unigene: 6870 Rat |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYL9) Polyclonal Antibody, Unconjugated (bs-2380R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
