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phospho-CD244 (Tyr271) Rabbit pAb (bs-5284R)  
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產品編號 bs-5284R
英文名稱 phospho-CD244 (Tyr271) Rabbit pAb
中文名稱 磷酸化CD244抗體
別    名 CD244(phospho Y271); CD244(phospho-Tyr271); NKR2B4; SLAMF4; natural killer cell receptor 2B4 isoform 2 precursor; 2B4; C9.1; CD244 antigen; CD244 molecule natural killer cell receptor 2B4; CD244 natural killer cell receptor 2B4; F730046O15Rik; h2B4; Ly90; NAIL; Natural killer cell activation-inducing ligand; Natural killer cell receptor 2B4; NK cell activation inducing ligand; NK cell activation inducing ligand NAIL; NK cell type I receptor protein 2B4; Nmrk; Non-MHC restricted killing associated; OTTHUMP00000027884; p38; SLAM family, member 4.  
Specific References  (1)     |     bs-5284R has been referenced in 1 publications.
[IF=4.047] Xu Xiaoyan. et al. A novel 2B4 receptor leads to worse pregnancy outcomes by facilitating TNF-α and IFN-γ production in dNK cells during Toxoplasma gondii infection. PARASITE VECTOR. 2022 Dec;15(1):1-15  WB ;  Human.  
產品類型 磷酸化抗體 
研究領域 腫瘤  免疫學  信號轉導  細胞凋亡  細胞表面分子  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human (predicted: Rabbit,Pig,Horse)
產品應用 WB=1:500-2000,Flow-Cyt=1ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 38 kDa
檢測分子量
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human CD244 around the phosphorylation site of Tyr271: TI(p-Y)ED 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 CD244 is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. CD244 activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness. Although there have been several molecules shown to interact with CD244, the signaling mechanism ofCD244-mediated activation of NK cells is still unknown. It is a high affinity receptor for CD48.

Function:
Modulate other receptor-ligand interactions to enhance leukocyte activation.

Subunit:
Interacts with CD48. Following phosphorylation, it is able to recruit PTPN11/SHP-2 and SH2D1A/SAP. Binding of SH2D1A/SAP to CD244 prevents its association with PTPN11/SHP-2.

Subcellular Location:
Membrane; Single-pass type I membrane protein (Potential).

Tissue Specificity:
Expressed in spleen, PBL, followed by lung, liver, testis and small intestine. Expressed not only in NK cells, but also on monocytes and basophils.

Similarity:
Contains 2 Ig-like (immunoglobulin-like) domains.

SWISS:
Q9BZW8

Gene ID:
51744

Database links:

Entrez Gene: 51744 Human

Entrez Gene: 18106 Mouse

Entrez Gene: 64025 Rat

Omim: 605554 Human

SwissProt: Q9BZW8 Human

SwissProt: Q07763 Mouse

SwissProt: Q9JLM2 Rat

Unigene: 157872 Human

Unigene: 2299 Mouse

Unigene: 113760 Rat

Unigene: 53994 Rat



產品圖片
Sample: Hela (Human) Lysate at 40 ug Primary: Anti- phospho-CD244(Tyr271) (bs-5284R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 65 kD
Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti-phospho-CD244(Tyr271) (bs-5284R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 70 kD
Blank control: U937. Primary Antibody (green line): Rabbit Anti-CD244 antibody (bs-5284R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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