97久久久精品综合88久久_亚洲国产精品一_久热热国产久热_97操操操_北条麻妃在线免费观看_精品国自产拍天天拍

掃碼關注公眾號           掃碼咨詢技術支持           掃碼咨詢技術服務
  
客服熱線:400-901-9800  客服QQ:4009019800  技術答疑  技術支持  質量反饋  關于我們  聯系我們
亚洲欧美国产日韩中文字幕,天堂va欧美va亚洲va好看va ,最新av免费在线
首頁 > 產品中心 > 一抗 > 產品信息
phospho-CDKN1B (Thr157) Rabbit pAb (bs-5228R)  
訂購熱線:400-901-9800
訂購郵箱:sales@bioss.com.cn
訂購QQ:  400-901-9800
技術支持:techsupport@bioss.com.cn
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包裝/詢價

產品編號 bs-5228R
英文名稱 phospho-CDKN1B (Thr157) Rabbit pAb
中文名稱 磷酸化P27抗體/周期素依賴激酶抑制劑抗體
別    名 p27 KIP 1(phospho T157); p-p27 KIP 1(phospho T157); p27Kip1(phospho-Thr157); p-p27Kip1(Thr157); p-p27Kip1(T157); CDN1B_HUMAN; AA408329; AI843786; Cdki1b; CDKN 1B; CDKN 4; CDKN1B; CDKN1B; CDKN4; CDKN4; Cyclin Dependent Kinase Inhibitor 1B; Cyclin Dependent Kinase Inhibitor 1B; Cyclin dependent kinase inhibitor p27; Cyclin dependent kinase inhibitor p27; Cyclin-dependent kinase inhibitor 1B(p27, Kip1); Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; Cyclin-dependent kinase inhibitor p27 Kip1; KIP 1; KIP1; MEN1B; MEN4; OTTHUMP00000195098; OTTHUMP00000195099; p27; p27 Kip1; P27-like cyclin-dependent kinase inhibitor; P27KIP1.  
產品類型 磷酸化抗體 
研究領域 免疫學  細胞周期蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat (predicted: Rabbit,Pig,Dog,GuineaPig)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 22 kDa
檢測分子量
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human CDKN1B around the phosphorylation site of Thr157: PA(p-T)DD 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq, Jul 2008]

Function:
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Subunit:
Forms a ternary compex with CCNE1/CDK2/CDKN1B.

Subcellular Location:
Nucleus. Cytoplasm. Endosome. Nuclear and cytoplasmic in quiescent cells.

Tissue Specificity:
Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

Post-translational modifications:
Phosphorylated; phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G(0)-G(1) phase and leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC and in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK2 leads to protein ubiquitination and proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 and Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF.
Ubiquitinated; in the cytoplasm by the KPC complex (composed of RNF123/KPC1 and UBAC1/KPC2) and, in the nucleus, by SCF(SKP2). The latter requires prior phosphorylation on Thr-187. Ubiquitinated; by a TRIM21-containing SCF(SKP2)-like complex; leads to its degradation.

DISEASE:
Defects in CDKN1B are the cause of multiple endocrine neoplasia type 4 (MEN4) . Multiple endocrine neoplasia (MEN) syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 and MEN2.

Similarity:
Belongs to the CDI family.

SWISS:
P46527

Gene ID:
1027

Database links:

Entrez Gene: 1027 Human

Entrez Gene: 12576 Mouse

Entrez Gene: 83571 Rat

Omim: 600778 Human

SwissProt: P46527 Human

Unigene: 238990 Human



P27蛋白是一種新發現的周期素依賴激酶抑制劑,屬于細胞周期的負性調控因子。P27基因及其產物的異常表達可能與某些腫瘤的發生、發展有著密切的關系。P27蛋白對細胞周期的調控及在腫瘤中發揮著很重要的作用。
產品圖片
Sample: Lane 1: Spleen (Mouse) Lysate at 40 ug Lane 2: Thymus (Mouse) Lysate at 40 ug Lane 3: Thymus (Rat) Lysate at 40 ug Primary: Anti-phospho-CDKN1B (Thr157) (bs-5228R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 25 kD
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-CDKN1B (Thr157)) Polyclonal Antibody, Unconjugated (bs-5228R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-CDKN1B (Thr157)) Polyclonal Antibody, Unconjugated (bs-5228R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control (blue line): MCF7(fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice) Primary Antibody (green line): Rabbit Anti-hospho-CDKN1B (Thr157) antibody (bs-5228R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
版權所有 2004-2026 www.kastlife.cn 北京博奧森生物技術有限公司
通過國際質量管理體系ISO 9001:2015 GB/T 19001-2016    證書編號: 00124Q34771R2M/1100
通過國際醫療器械-質量管理體系ISO 13485:2016 GB/T 42061-2022    證書編號: CQC24QY10047R0M/1100
京ICP備05066980號-1         京公網安備110107000727號
主站蜘蛛池模板: 午夜精品成人 | a级免费观看视频 | 亚洲欧美综合视频 | 国产二级一片内射视频播放 | 日韩美一区 | 狠狠干人人干 | 精品日产一区二区三区手机 | 69精产国品一二三产品价格 | 插插插干干干 | 好大好爽好深国产在线 | 欧美视频免费在线 | 免费亚洲视频在线观看 | 最好看的中文视频最好的中文 | 国产精品色区 | 91国产一区二区 | 国产成人久久精品激情91 | 亚洲欧美日韩一区二区在线观看 | 少妇乳大丰满在线播放 | 久久精久久 | 精品人妻无码一区二区三区丝袜 | 婷婷亚洲综合小说图片 | A级国产乱理论片在线观看 亚洲激情第一页 | 国产精品无码无卡无需播放器 | 久久精品水蜜桃AV综合天堂 | 免费在线91 | 国产亚洲综合精品 | 久久不见久久见中文字幕免费 | 亚洲日韩中文字幕天堂不卡 | 最近免费日本视频在线 | 日本老妇性视频 | 九九在线免费视频 | 成人国产一区二区三区精品麻豆 | 日韩欧美毛片免费观看 | 欧美性猛交xxxx乱大交免费看 | 在线看毛片的网站 | 亚洲精品无码久久久久秋霞 | 亚洲中文无码成人手机版 | 欧美男女爱爱视频 | 色悠综合 | 人人爽人人爽人人爽人人片av | 国产成人午夜片在线观看高清观看 |