| 產品編號 | bs-4195R |
| 英文名稱 | ADAM8 Rabbit pAb |
| 中文名稱 | 去整合素樣金屬蛋白酶8抗體 |
| 別 名 | A Disintegrin And Metalloproteinase domain 8; A Disintegrin And Metalloproteinase domain 8; ADAM 8; ADAM 8 precursor; ADAM 8 precursor; ADAM metallopeptidase domain 8; ADAM8 protein; CD 156; CD156; CD156a; CD156a antigen; CD156a antigen; Cell surface antigen MS2; Cell surface antigen MS2; Human leukocyte differentiation antigen; ADAM8_HUMAN; Human leukocyte differentiation antigen; Macrophage cysteine rich glycoprotein; Macrophage cysteine rich glycoprotein; MGC134985; MS 2; MS2. |
| 研究領域 | 細胞生物 免疫學 信號轉導 細胞凋亡 細胞骨架 細胞外基質 泛素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse (predicted: Rat,Cow,Dog) |
| 產品應用 | Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 87 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ADAM8 52-91aa: 51-150/824 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Members of ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. The extracellular region of ADAM8 shows significant amino acid sequence homology to hemorrhagic snake venom proteins, including the metalloprotease and disintegrin domains. The expression of ADAM8 is upregulated by retinoic acid and vitamin D3. Function: Possible involvement in extravasation of leukocytes. Subunit: Interacts with FST3. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed on neutrophils and monocytes. Similarity: Contains 1 disintegrin domain. Contains 1 EGF-like domain. Contains 1 peptidase M12B domain. SWISS: P78325 Gene ID: 101 Database links: Entrez Gene: 101 Human Entrez Gene: 11501 Mouse Omim: 602267 Human SwissProt: P78325 Human SwissProt: Q05910 Mouse Unigene: 501574 Human Unigene: 15969 Mouse 細胞外基質蛋白 |
| 產品圖片 |
Blank control: Mouse splenocytes(blue)
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).
Black line : Positive blank control A431); Negative blank control (HepG2）
Green line : Primary Antibody (Rabbit Anti-ADAM8 antibody (bs-4195R) )
Orange line：Isotype Control Antibody (Rabbit IgG) .
Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF488)
A431（Positive）and HepG2（Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ADAM8 Antibody(bs-4195R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-ADAM8 antibody (bs-4195R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
