| 產(chǎn)品編號 | bs-6110R |
| 英文名稱 | SPARCL1 Rabbit pAb |
| 中文名稱 | 細胞外基質(zhì)蛋白2抗體 |
| 別 名 | Ecm2; SPRL1; RAGS1 antigen; Extracellular matrix protein 2; Hevin; High endothelial venule protein; MAST 9; mast9; Matrix glycoprotein Sc1; PIG33; proliferation inducing protein 33; SC1; SPARC like 1; SPARC-like protein 1; SPRL1_HUMAN. |
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Specific References (3) | bs-6110R has been referenced in 3 publications.
[IF=5.959] Wang Y et al. SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway. Cell Death Dis. 2019 Nov 7;10(11):852. IHF&WB ; Mouse.
[IF=3.9] Wallingford et al. Altered Developmental Expression of the Astrocyte-Secreted Factors Hevin and SPARC in the Fragile X Mouse Model. (2017) Front.Mol.Neurosci. 10:268 ICC,WB ; Mouse.
[IF=0] "Wallingford, J. (2016). Astrocyte-Secreted Factors in Fragile X Mice (Doctoral dissertation).Diss. WB, IF(ICC) ; Mouse.
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| 研究領(lǐng)域 | 腫瘤 鋅指蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 74 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SPARCL1/Ecm2: 601-664/664 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
SPARC (secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. SPARC-like protein 1 (SPARCL1), also known as high endothelial venule protein (Hevin) or MAST9, is a 664 amino acid member of the SPARC family of proteins. Highly expressed in lymph node, heart, lung, brain, skeletal muscle, ovary, colon and small intestine, SPARCL1 is a secreted protein that contains one EF-hand domain, one follistatin-like domain and one Kazal-like domain. SPARCL1 is implicated to play a role in neuronal remodeling and tumor suppression. The gene encoding SPARCL1 maps to chromosome 4q22.1. Subcellular Location: Secreted, extracellular space, extracellular matrix. Tissue Specificity: Highly expressed in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon, with lower levels in placenta, pancreas, testis, spleen, and thymus, and no expression in kidney, liver, and peripheral blood leukocytes. Post-translational modifications: N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. Thr-398 is the preferred site to Thr-399. Similarity: Belongs to the SPARC family. Contains 1 EF-hand domain. Contains 1 follistatin-like domain. Contains 1 Kazal-like domain. SWISS: Q14515 Gene ID: 8404 Database links: Entrez Gene: 8404 Human Omim: 606041 Human SwissProt: Q14515 Human Unigene: 62886 Human |
| 產(chǎn)品圖片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-SPARCL1 (bs-6110R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 74 kD
Observed band size: 73 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (SPARCL1) Polyclonal Antibody, Unconjugated (bs-6110R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
