| 產(chǎn)品編號 | bs-5841R |
| 英文名稱 | MGAT5 Rabbit pAb |
| 中文名稱 | 糖蛋白GNTVA抗體 |
| 別 名 | 6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A; 6-N-acetylglucosaminyltransferase; alpha 1, 3(6) mannosylglycoprotein; alpha 1, 6 mannosylglycoprotein 6 beta N acetylglucosaminyltransferase; alpha mannoside beta 1, 6 N acetylglucosaminyltransferase; alpha mannoside beta 1, 6 N acetylglucosaminyltransferase; alpha-mannoside beta-1, 6-N-acetylglucosaminyltransferase; alpha-1; alpha-1, 3(6)-mannosylglycoprotein; alpha-1, 6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase; alpha-mannoside beta-1; alpha-mannoside beta-1, 6-N-acetylglucosaminyltransferase; beta 1, 6 N acetyl glucosaminyltransferase; beta-1, 6-N-acetyl-glucosaminyltransferase; GGNT5; GlcNAc T V; GlcNAc-T V; GNT V; GNT VA; GNT-V; GNTV; GNTVA; Mannoside acetylglucosaminyltransferase 5; Mannosyl(alpha 1, 6) glycoprotein beta 1, 6 N acetyl glucosaminyltransferase; mannosyl(alpha-1, 6-)-glycoprotein beta-1, 6-N-acetyl-glucosaminyltransferase; Mgat5; MGT5A_HUMAN; N acetylglucosaminyl transferase V; N acetylglucosaminyltransferase V mannosyl(alpha 1, 6) glycoprotein; N-acetylglucosaminyl-transferase V; N-acetylglucosaminyltransferase V. |
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Specific References (1) | bs-5841R has been referenced in 1 publications.
[IF=3.375] Yang P et al. Adoptive transfer of polarized M2c macrophages ameliorates acute rejection in rat liver transplantation. Am J Transl Res. 2020 Jun 15;12(6):2614-2626. WB ; Rat.
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| 研究領域 | 腫瘤 細胞生物 免疫學 信號轉導 細胞粘附分子 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Chicken,Dog) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 85 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MGAT5: 201-300/741 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
MGAT5 is a mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, a glycosyltransferase involved in the synthesis of protein-bound and lipid-bound oligosaccharides. Alterations of the oligosaccharides on cell surface glycoproteins cause significant changes in the adhesive or migratory behavior of a cell. Increase in the encoded protein's activity may correlate with the progression of invasive malignancies. Function: Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. Subcellular Location: Golgi apparatus membrane; Single-pass type II membrane protein. Similarity: Belongs to the glycosyltransferase 18 family. SWISS: Q09328 Gene ID: 4249 Database links: Entrez Gene: 4249 Human Entrez Gene: 107895 Mouse Omim: 601774 Human SwissProt: Q09328 Human SwissProt: Q8R4G6 Mouse Unigene: 651869 Human Unigene: 214682 Mouse Unigene: 391592 Mouse Unigene: 472488 Mouse Unigene: 53998 Rat |
| 產(chǎn)品圖片 |
Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti- MGAT5 (bs-5841R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 85 kD
Observed band size: 85 kD
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MGAT5 ) Polyclonal Antibody, Unconjugated (bs-5841R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MGAT5 ) Polyclonal Antibody, Unconjugated (bs-5841R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
