| 產品編號 | bs-5888R |
| 英文名稱 | Hyaluronidase2 Rabbit pAb |
| 中文名稱 | 透明質酸酶2/玻璃酸酶2抗體 |
| 別 名 | Hyal 2; Hyal-2; Hyal2; HYAL2_HUMAN; Hyaluronidase 2; Hyaluronidase 2 Precursor; Hyaluronidase-2; Hyaluronoglucosaminidase 2; Hyaluronoglucosaminidase-2; LUCA 2; LUCA-2; LUCA2; Lung carcinoma protein 2; lysosomal hyaluronidase; PH 20 homolog. |
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Specific References (2) | bs-5888R has been referenced in 2 publications.
[IF=6.438] Anna Kocurkova. et al. Endogenously produced hyaluronan contributes to the regulation of peritoneal adhesion development. BIOFACTORS. 2023 May;: IHC ; Mouse.
[IF=4.879] Petra ?ádníková. et al. The Degradation of Hyaluronan in the Skin. Biomolecules. 2022 Feb;12(2):251 WB,IHC,IF ; Human.
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| 研究領域 | 腫瘤 細胞生物 免疫學 信號轉導 細胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse (predicted: Rat) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 49 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HYAL2: 111-210/473 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R. Function: Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R. Subunit: Interacts with MST1R. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Tissue Specificity: Widely expressed. No expression detected in adult brain. Similarity: Belongs to the glycosyl hydrolase 56 family. Contains 1 EGF-like domain. SWISS: Q12891 Gene ID: 8692 Database links: Entrez Gene: 8692 Human Entrez Gene: 15587 Mouse Omim: 603551 Human SwissProt: Q12891 Human SwissProt: O35632 Mouse Unigene: 76873 Human Unigene: 440982 Mouse Unigene: 4834 Mouse Unigene: 6149 Pig Unigene: 8278 Rat |
| 產品圖片 |
Sample: Testis (Mouse) Lysate at 40 ug
Primary: Anti-Hyaluronidase2 (bs-5888R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Hyluronidase 2 Polyclonal Antibody, Unconjugated(bs-5888R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
