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Hyaluronidase3 Rabbit pAb (bs-5889R)  
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產品編號 bs-5889R
英文名稱 Hyaluronidase3 Rabbit pAb
中文名稱 透明質酸酶3/玻璃酸酶3抗體
別    名 Hyaluronidase 3; HYAL3; Hyaluronidase-3; Hyaluronoglucosaminidase 3; LUCA14; LUCA3; Minna14; HYAL3_HUMAN.  
研究領域 腫瘤  細胞生物  免疫學  信號轉導  細胞表面分子  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat (predicted: Pig,Sheep)
產品應用 WB=1:500-2000,Flow-Cyt=2ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 47 kDa
檢測分子量
細胞定位 細胞漿 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Hyaluronidase3: 51-150/417 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 HYAL3 is a protein which is similar in structure to hyaluronidases. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. However, this protein has not yet been shown to have hyaluronidase activity.

Subcellular Location:
Secreted (By similarity). Lysosome (By similarity).

Tissue Specificity:
Bone marrow, testis and kidney. Isoform 4 is detected in all bladder tumor and prostate tumor cells.

Similarity:
Belongs to the glycosyl hydrolase 56 family.
Contains 1 EGF-like domain.

SWISS:
O43820

Gene ID:
8372

Database links:

Entrez Gene: 8372 Human

Entrez Gene: 109685 Mouse

Entrez Gene: 300993 Rat

Omim: 604038 Human

SwissProt: O43820 Human

SwissProt: Q8VEI3 Mouse

SwissProt: Q76HM9 Rat

Unigene: 129910 Human

Unigene: 729310 Human

Unigene: 447916 Mouse

Unigene: 72927 Rat



產品圖片
Sample: Mouse(Kidney) Lysate at 40 ug Primary: Anti- Hyaluronidase3 (bs-5889R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 48 kD
Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Stomach tissue lysates Lane 3: Rat Cerebrum tissue lysates Lane 4: Rat Stomach tissue lysates Primary: Anti-Hyaluronidase3 (bs-5889R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 53 kDa
Blank control:Molt4. Primary Antibody (green line): Rabbit Anti-Hyaluronidase3 antibody (bs-5889R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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