| 產品編號 | bs-6003R |
| 英文名稱 | DAL1/EPB41L3 Rabbit pAb |
| 中文名稱 | 細胞骨架4.1蛋白家族DAL1抗體 |
| 別 名 | Differentially expressed in adenocarcinoma of the lung protein 1; 4.1B; Band 4.1 like protein 3; DAL 1; DAL1; EPB41L3; Erythrocyte membrane protein band 4.1 like 3; HGNC:3380; KIAA0987; E41L3_HUMAN. |
| 研究領域 | 腫瘤 細胞生物 免疫學 神經生物學 細胞骨架 細胞膜蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 120 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DAL1/EPB41L3: 131-230/1087 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
EPB41L3 is an actin-binding protein originally identified as a molecule whose expression is down-regulated in lung adenocarcinoma. EPB41L3 is a member of the protein 4.1 superfamily, which encompasses structural proteins that play important roles in membrane processes via interactions with actin, spectrin, and the cytoplasmic domains of integral membrane proteins. The gene for EPB41L3 is thought to function as a tumor suppressor and localizes within chromosomal region 18p11.3, which is affected by loss of heterozygosity (LOH) in various adult tumors. Function: Critical growth regulator in the pathogenesis of meningiomas. Subunit: Interacts with CADM1. Subcellular Location: Cytoplasm, cytoskeleton Tissue Specificity: Expressed at high levels in brain, with lower levels in kidney, intestine, and testis. Similarity: ontains 1 FERM domain. SWISS: Q9Y2J2 Gene ID: 23136 Database links: Entrez Gene: 23136 Human Entrez Gene: 13823 Mouse Omim: 605331 Human SwissProt: Q9Y2J2 Human SwissProt: Q9WV92 Mouse Unigene: 213394 Human Unigene: 131135 Mouse |
| 產品圖片 |
Sample:
Lane 1: Human HUVEC cell lysates
Lane 2: Human U-2 OS cell lysates
Primary: Anti-DAL1/EPB41L3 (bs-6003R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 120 kDa
Observed band size: 120 kDa
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DAL1(EPB41L3)) Polyclonal Antibody, Unconjugated (bs-6003R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DAL1(EPB41L3)) Polyclonal Antibody, Unconjugated (bs-6003R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
