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RXR beta Rabbit pAb (bs-6219R)  
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產品編號 bs-6219R
英文名稱 RXR beta Rabbit pAb
中文名稱 核受體RXRβ抗體
別    名 Retinoid X Receptor beta; DAUD I6; DAUDI6; H 2RIIBP; H2RIIBP; MHC class I promoter binding protein; NR2B2; RCoR 1; RCoR1; Retinoic acid receptor RXR beta; RXR beta; RXRB; RXRbeta.  
研究領域 腫瘤  細胞生物  染色質和核信號  信號轉導  轉錄調節因子  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse (predicted: Rat,Pig,Cow,Dog)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 59 kDa
檢測分子量
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human RXR beta: 201-300/533 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the effects of retinoic acid (RA). The encoded protein forms homodimers with the retinoic acid, thyroid hormone, and vitamin D receptors, increasing both DNA binding and transcriptional function on their respective response elements. This gene lies within the major histocompatibility complex (MHC) class II region on chromosome 6. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012].

Function:
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. Specifically binds 9-cis retinoic acid (9C-RA).

Subunit:
Homodimer. Heterodimer with a RAR molecule. Binds DNA preferentially as a RAR/RXR heterodimer.

Subcellular Location:
Nucleus.

Tissue Specificity:
Expressed in a variety of tumor cell lines.

Similarity:
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain.

SWISS:
P28702

Gene ID:
6257

Database links:

Entrez Gene: 6257 Human

Entrez Gene: 20182 Mouse

Omim: 180246 Human

SwissProt: P28702 Human

SwissProt: P28704 Mouse

Unigene: 388034 Human

Unigene: 1243 Mouse



產品圖片
Sample: Spleen (Mouse) Lysate at 40 ug Heart (Mouse) Lysate at 40 ug Primary: Anti-RXR beta (bs-6219R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 65 kD
Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RXRB) Polyclonal Antibody, Unconjugated (bs-6219R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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