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Hepsin/TMPRSS1 Rabbit pAb (bs-6281R)  
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產品編號 bs-6281R
英文名稱 Hepsin/TMPRSS1 Rabbit pAb
中文名稱 跨膜蛋白酶絲氨酸1抗體
別    名 HEPS; HEPS_HUMAN; Hepsin; HPN; Serine protease hepsin; Serine protease hepsin catalytic chain; Serine protease hepsin non catalytic chain; TMPRSS 1; TMPRSS1; TMPRSS-1; Transmembrane protease serine 1.  
研究領域 腫瘤  細胞生物  信號轉導  腫瘤細胞生物標志物  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human (predicted: Mouse,Rat,Rabbit,Pig,Sheep,Cow)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 28 kDa
檢測分子量
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Hepsin: 311-417/417 <Extracellular>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes a type II transmembrane serine protease. The encoded protein has an extracellular region that consists of two domains, a catalytic serine protease domain and a non-catalytic scavenger receptor cysteine-rich domain. This protein may be involved in diverse cellular functions including blood coagulation, maintenance of cell morphology and the growth and progression of certain cancers, particularly prostate cancer. Alternative splicing results in multiple transcript variants.

Function:
Plays an essential role in cell growth and maintenance of cell morphology. May mediate the activating cleavage of HGF and MST1/HGFL.

Subcellular Location:
Membrane; Single-pass type II membrane protein.

Tissue Specificity:
Present in most tissues, with the highest level in liver.

Similarity:
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 1 SRCR domain.

SWISS:
P05981

Gene ID:
3249

Database links:

Entrez Gene: 3249 Human

Omim: 142440 Human

SwissProt: P05981 Human

Unigene: 182385 Human



產品圖片
Sample: HepG2 (human)Cell Lysate at 40 ug Primary: Anti- Hepsin/TMPRSS1 (bs-6281R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28kD Observed band size: 28 kD
Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Hepsin Polyclonal Antibody, Unconjugated(bs-6281R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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