| 產品編號 | bs-6287R |
| 英文名稱 | UEVLD Rabbit pAb |
| 中文名稱 | 泛素結合酶E2樣蛋白抗體 |
| 別 名 | ATTP; Ubiquitin-conjugating enzyme E2 variant 3; EV and lactate malate dehydrogenase domain containing protein; EV and lactate/malate dehydrogenase domain-containing protein; signaling molecule ATTP; ubiquitin conjugating enzyme E2 like; Ubiquitin conjugating enzyme E2 variant 3; Ubiquitin E2 variant and lactate/malate dehydrogenase domain containing protein; Ubiquitin-conjugating enzyme E2 variant 3; UEV 3; UEV and lactate malate dehyrogenase domains; UEV-3; UEV2 and LDH domains containing protein; UEV3; uevld; UEVLD_HUMAN. |
| 研究領域 | 腫瘤 細胞生物 信號轉導 細胞周期蛋白 腫瘤細胞生物標志物 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Rat (predicted: Human,Mouse,Rabbit,Pig,Cow,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 52 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞外基質 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human UEVLD: 85-180/471 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
UEV3, also known as EV and lactate/malate dehydrogenase domain-containing protein, is a 471 amino acid protein that contains one UEV (ubiquitin E2 variant) domain, which typically interacts with ubiquitin. UEV3 is thought to be a paralogue of tsg 101, a protein that exerts regulatory effects on E2 activity in cellular ubiquitination processes. With amino-terminal homology to the catalytic domain of ubiquitin-conjugating enzymes, it is thought that UEV3 may function as a negative regulator of polyubiquitination. UEV3 is expressed in various colon carcinoma cell lines, carcinomas of the uterine cervix and peripheral blood leukocytes as well as normal colon and cervical epithelium. Subunit: Homodimer. Tissue Specificity: Colon, colon carcinoma cell lines, normal cervical epithelium, carcinomas of the uterine cervix and peripheral blood leukocytes. Similarity: In the N-terminal section; belongs to the ubiquitin-conjugating enzyme family. UEV subfamily. In the C-terminal section; belongs to the LDH/MDH superfamily. Contains 1 UEV (ubiquitin E2 variant) domain. SWISS: Q8IX04 Gene ID: 55293 Database links: Entrez Gene: 55293 Human Omim: 610985 Human SwissProt: Q8IX04 Human Unigene: 407991 Human |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UEVLD) Polyclonal Antibody, Unconjugated (bs-6287R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
