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CAT Rabbit pAb (bs-6874R)  
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50ul/1180.00元
100ul/1980.00元
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產(chǎn)品編號(hào) bs-6874R
英文名稱(chēng) CAT Rabbit pAb
中文名稱(chēng) 過(guò)氧化氫酶抗體
別    名 CATA_HUMAN; Catalase; EC:1.11.1.6.  
Specific References  (9)     |     bs-6874R has been referenced in 9 publications.
[IF=15.304] Yao Lei. et al. Phytochemical natural killer cells reprogram tumor microenvironment for potent immunotherapy of solid tumors. BIOMATERIALS. 2022 Jun;:121635  IF, FC ;  Mouse.  
[IF=8.897] Liu, Houli. et al. Immunomodulatory hybrid bio-nanovesicle for self-promoted photodynamic therapy. Nano Res. 2022 Feb;:1-10  WB ;  Mouse.  
[IF=7.675] Shuang Wang. et al. Dioscin Protects against Cisplatin-Induced Acute Kidney Injury by Reducing Ferroptosis and Apoptosis through Activating Nrf2/HO-1 Signaling. ANTIOXIDANTS-BASEL. 2022 Dec;11(12):2443  IHC ;  Rat.  
[IF=7.468] Dr. Evan Paul Tracy. et al. Stromal Vascular Fraction Restores Vasodilatory Function by Reducing Oxidative Stress in Aging-Induced Coronary Microvascular Disease. ANTIOXID REDOX SIGN. 2022 Aug 11  IF ;  Rat.  
[IF=6.1] Yun, Yang, et al. "Sulfate Aerosols Promote Lung Cancer Metastasis by Epigenetically Regulating the Epithelial-to-Mesenchymal Transition (EMT)." Environmental Science & Technology (2017).  WB ;  Human.  
[IF=4.868] Wang PW et al. Red Raspberry Extract Protects the Skin against UVB-Induced Damage with Antioxidative and Anti-inflammatory Properties. Oxid Med Cell Longev.?2019 Jan 6;2019:9529676.  WB ;  Human.  
[IF=3.69] Sheng Zhang. et al. Proteomics analysis in the kidney of mice following oral feeding Realgar. J Ethnopharmacol. 2021 Jul;275:114118  WB ;  Mouse.  
[IF=3.47] Minjeong Kim. et al. Ibuprofen Increases the Hepatotoxicity of Ethanol through Potentiating Oxidative Stress. Biomol Ther. 2021 Mar 1; 29(2): 205–210  WB ;  Human.  
[IF=2.323] Liu R et al. Effect of Dietary Methionine Deficiency Followed by a Re-Feeding Phase on the Hepatic Antioxidant Activities of LambsAnimals (Basel).2020 Dec 23;11(1):7.  WB ;  Lamb.  
研究領(lǐng)域 腫瘤  細(xì)胞生物  免疫學(xué)  發(fā)育生物學(xué)  神經(jīng)生物學(xué)  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Human,Rat (predicted: Mouse,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 60 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse Catalase 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Catalase is the classical marker for peroxisomes and is the most abundant protein within peroxisomes. It is found in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.

Function:
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.

Subunit:
Homotetramer.

Subcellular Location:
Peroxisome.

Post-translational modifications:
The N-terminus is blocked.

DISEASE:
Defects in CAT are the cause of acatalasemia (ACATLAS) [MIM:614097]. A metabolic disorder characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.

Similarity:
Belongs to the catalase family.

SWISS:
P24270

Gene ID:
12359

Database links:

Entrez Gene: 847 Human

Entrez Gene: 12359 Mouse

Entrez Gene: 24248 Rat

Omim: 115500 Human

SwissProt: P04040 Human

SwissProt: P24270 Mouse

SwissProt: P04762 Rat

Unigene: 502302 Human

Unigene: 4215 Mouse

Unigene: 3001 Rat



過(guò)氧化氫酶是催化過(guò)氧化氫分解成氧和水的酶,存在于細(xì)胞的過(guò)氧化物體內(nèi)。過(guò)氧化氫酶是過(guò)氧化物酶體的標(biāo)志酶, 約占過(guò)氧化物酶體酶總量的40%。過(guò)氧化氫酶存在于所有已知的動(dòng)物的各個(gè)組織中,特別在肝臟中以高濃度存在。過(guò)氧化氫酶在食品工業(yè)中被用于除去用于制造奶酪的牛奶中的過(guò)氧化氫。過(guò)氧化氫酶也被用于食品包裝,防止食物被氧化。
產(chǎn)品圖片
Sample: Lane 1: Rat Kidney tissue lysates Lane 2: Rat Placenta tissue lysates Lane 3: Rat Heart tissue lysates Lane 4: Human HeLa cell lysates Lane 5: Human HepG2 cell lysates Primary: Anti-CAT (bs-6874) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kDa Observed band size: 60 kDa
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Catalase Polyclonal Antibody, Unconjugated(bs-6874R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: HL60. Primary Antibody (green line): Rabbit Anti-Catalase antibody (bs-6874R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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