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PAG608 Rabbit pAb (bs-0957R)  
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產(chǎn)品編號(hào) bs-0957R
英文名稱 PAG608 Rabbit pAb
中文名稱 野生型P53誘導(dǎo)基因1抗體
別    名 WIG-1; p53 activated gene 608; PAG608; WIG1; FLJ12296; MGC10613; OTTHUMP00000211906; OTTHUMP00000211907; p53-activated gene 608 protein; PAG 608; WIG 1; Zinc finger matrin-type protein 3; Zinc finger protein WIG-1; ZMAT3_HUMAN; p53 inducible zinc finger protein; p53 target zinc finger protein; PAG 608; WIG 1; WIG 1/PAG608 protein; FLJ31683; WIG1; Zinc finger matrin-type protein 3; WIG1; WIG1/PAG608 protein; Zinc finger matrin type 3; Zinc finger protein WIG 1; Zinc finger protein WIG1; ZMAT 3; ZMAT3.  
研究領(lǐng)域 腫瘤  細(xì)胞生物  染色質(zhì)和核信號(hào)  信號(hào)轉(zhuǎn)導(dǎo)  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Human,Rat (predicted: Mouse,Cow,Chicken,Dog,GuineaPig,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 32 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human WIG-1: 51-150/289 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a protein containing three zinc finger domains and a nuclear localization signal. The mRNA and the protein of this gene are upregulated by wildtype p53 and overexpression of this gene inhibits tumor cell growth, suggesting that this gene may have a role in the p53-dependent growth regulatory pathway. Alternative splicing of this gene results in two transcript variants encoding two isoforms differing in only one amino acid.

Function:
Acts as a bona fide target gene of p53/TP53. May play a role in the TP53-dependent growth regulatory pathway. May contribute to TP53-mediated apoptosis by regulation of TP53 expression and translocation to the nucleus and nucleolus.

Subunit:
Interacts with dsRNA.

Subcellular Location:
Nucleus. Nucleus, nucleolus.

Tissue Specificity:
Highly expressed in adult brain, and moderately in adult kidney and testis. Not detected in fetal brain, heart, pancreas, adrenal gland, liver or small intestine.

Similarity:
Contains 3 matrin-type zinc fingers.

SWISS:
Q9HA38

Gene ID:
64393

Database links:

Entrez Gene: 64393 Human

Entrez Gene: 22401 Mouse

Entrez Gene: 64394 Rat

Omim: 606452 Human

SwissProt: Q9HA38 Human

SwissProt: O54836 Mouse

SwissProt: O08781 Rat

Unigene: 371609 Human

Unigene: 35705 Mouse

Unigene: 2925 Rat



Tissue Specificity : Highly expressed in adult brain, and moderately in adult kidney and testis. Not detected in fetal brain, heart, pancreas, adrenal gland, liver or small intestine.
產(chǎn)品圖片
Sample: Lane 1: 293T (Human) Cell Lysate at 30 ug Lane 2: Huvec (Human) Cell Lysate at 30 ug Lane 3: U87MG (Human) Cell Lysate at 30 ug Lane 4: U2os (Human) Cell Lysate at 30 ug Primary: Anti- PAG608 (bs-0957R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 32 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PAG608) Polyclonal Antibody, Unconjugated (bs-0957R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PAG608) Polyclonal Antibody, Unconjugated (bs-0957R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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