| 產品編號 | bs-11803R |
| 英文名稱 | GPR10 Rabbit pAb |
| 中文名稱 | G蛋白偶聯受體10抗體 |
| 別 名 | G protein coupled receptor 10; G-protein coupled receptor 10; GPR 10; GPR10; GCPR 10; GR 3; GR3; hGR 3; hGR3; MGC126539; MGC126541; PRLH RECEPTOR; PRLHR; PRLHR_HUMAN; Prolactin releasing hormone receptor; Prolactin releasing peptide receptor; Prolactin-releasing peptide receptor; PrRP receptor; PrRPR; uhr-1. |
| 研究領域 | 神經生物學 信號轉導 G蛋白偶聯受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Dog,Monkey) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 41 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPR10 protein: 161-260/370 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
G protein-coupled receptors (GPRs or GPCRs), also known as seven transmembrane receptors, heptahelical receptors, or 7TM receptors, are members of the largest protein family and play a role in many different stimulus-response pathways. G-protein coupled receptors mediate extracellular signals into intracellular signals (G-protein activation). They respond to a great variety of signaling molecules, including hormones, neurotransmitters and other proteins and peptides. GPR proteins are integral seven-pass membrane proteins with some conserved amino acid regions. G-protein coupled receptor 10 (GPR10) acts as a receptor for prolactin-releasing peptide (PrRP). GPR10 plays a role in the regulation of food intake, pain-signal processing and lactation. Primarily expressed in pituitary gland, it is repressed by bromocriptine. GPR10 interacts with various other proteins, including GRIP1, GRIP2 and PICK1. Function: Receptor for prolactin-releasing peptide (PrRP). Implicated in lactation, regulation of food intake and pain-signal processing. Subunit: Interacts through its C-terminal region with the PDZ domain-containing proteins GRIP1, GRIP2 and PICK1. Interacts with PDZ domains 4 and 5 of GRIP1 and with the PDZ domain of PICK1. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Only detected in the pituitary gland and in all cell types of pituitary adenomas. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P49683 Gene ID: 2834 Database links: Entrez Gene: 2834 Human Entrez Gene: 100412185 Marmoset (common) Entrez Gene: 226278 Mouse Omim: 600895 Human SwissProt: P49683 Human SwissProt: Q6VMN6 Mouse Unigene: 248119 Human Unigene: 377241 Mouse Unigene: 138127 Rat |
| 產品圖片 |
Sample:
Lane 1: Mouse Pituitary tissue lysates
Lane 2: Mouse Muscle tissue lysates
Lane 3: Rat Muscle tissue lysates
Primary: Anti-GPR10 (bs-11803R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 52 kDa
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- GPR10 (bs-11803R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 51 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
