| 產品編號 | bs-12014R |
| 英文名稱 | GRM1 + GRM5 Rabbit pAb |
| 中文名稱 | 促代謝型谷氨酸受體1+5抗體 |
| 別 名 | MGLUR1+MGLUR5; GPRC1A; GPRC1E; GRM1A; Metabotropic glutamate receptor 1; Metabotropic glutamate receptor 5; mGlu1; mGlu5; MGLUR1; MGLUR1A; MGLUR5; MGLUR5B; GRM1_HUMAN; GRM5_HUMAN. |
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Specific References (1) | bs-12014R has been referenced in 1 publications.
[IF=2.666] X Zhou et al. Modulating NMDA receptors to treat MK-801-induced schizophrenic cognition deficit: effects of clozapine combining with PQQ treatment and possible mechanisms of action. BMC Psychiatry. 2020 Mar 6;20(1):106. WB ; rat.
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| 研究領域 | 細胞生物 神經生物學 信號轉導 合成與降解 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse (predicted: Human,Rat,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 130 kDa |
| 檢測分子量 | 140-150 |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MGLUR1 + MGLUR5: 501-600/1194 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Glutamate is the main excitatory neurotransmitter in the brain. For many years it had been considered to act only on the ligand-gated receptor channels-termed NMDA, AMPA and kainite receptors that are involved in the fast excitatory synaptic transmission. Recently, glutamate has been shown to regulate enzymes producing second messengers via specific receptors coupled to G-proteins. These receptors are called metabotropic glutamate receptors. In expression systems, Group-I receptors stimulate phospholipase C as revealed by an increase in phosphoinositide turnover and calcium release from internal stores. Group-II and -III receptors are coupled to the inhibition of adenylyl cyclase. The Group-I receptors include Metabotropic Glutamate Receptor 5 and Metabotropic Glutamate Receptor 1a. The Group-II receptors in include mGluR2 and mGluR3. Function: Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum. Subunit: Homodimer; disulfide-linked. The PPXXF motif binds HOMER1, HOMER2 and HOMER3. Interacts with SIAH1, RYR1, RYR2, ITPR1, SHANK1, SHANK3 and GRASP Subcellular Location: Cell membrane; Multi-pass membrane protein. Similarity: Belongs to the G-protein coupled receptor 3 family. SWISS: Q13255 Gene ID: 2911 Database links: Entrez Gene: 2911 Human Entrez Gene: 14816 Mouse SwissProt: Q13255 Human SwissProt: P97772 Mouse |
| 產品圖片 |
Sample:
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti- MGLUR1 + MGLUR5 (bs-12014R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 150 kD
Sample:
Hippocampus (Mouse) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- MGLUR1 + MGLUR5 (bs-12014R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 150 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
