| 產品編號 | bs-12561R |
| 英文名稱 | FLRT2 Rabbit pAb |
| 中文名稱 | 富含亮氨酸跨膜纖連蛋白2抗體 |
| 別 名 | Fibronectin leucine rich transmembrane protein 2; Fibronectin-like domain-containing leucine-rich transmembrane protein 2; KIAA0405; Leucine rich repeat transmembrane protein; Leucine rich repeat transmembrane protein FLRT2; FLRT2_HUMAN. |
| 研究領域 | 細胞生物 發育生物學 干細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 70 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FLRT2: 201-300/660 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
FLRT2 is a 660 amino acid single-pass type I membrane protein that is expressed in pancreas, skeletal muscle, brain and heart. Comprised of one fibronectin type-III domain and ten LRR (leucine-rich) repeats, FLRT2 may play a role in cell adhesion and/or receptor signaling. It is suggested that FLRT2 is involved in mediating events such as NCC (neural crest cell) migration, chondrogenesis and epithelial-mesenchymal interactions during craniofacial development. Function: May have a function in cell adhesion and/or receptor signaling. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed in pancreas, skeletal muscle, brain, and heart. Post-translational modifications: N-glycosylated. Similarity: Contains 1 fibronectin type-III domain. Contains 10 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain. SWISS: O43155 Gene ID: 23768 Database links: Entrez Gene: 23768 Human Entrez Gene: 399558 Mouse Omim: 604807 Human SwissProt: O43155 Human Unigene: 533710 Human Unigene: 341948 Mouse |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FLRT2) Polyclonal Antibody, Unconjugated (bs-12561R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FLRT2 Polyclonal Antibody, Unconjugated(bs-12561R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
