| 產品編號 | bs-0220P |
| 英文名稱 | Alkaline Phosphatase(≥5,500 U/mg) |
| 中文名稱 | 堿性磷酸酶 (進口分裝 ≥5,500 U/mg)蛋白 |
| 別 名 | Alkaline Phosphatase from calf intestine; Phosphatase, Alkaline from bovine intestinal mucosa; Alkaline phosphatase; Orthophosphoric-monoester phosphohydrolase (alkaline optimum); ALP-1; ALP; ALPP; ALPPL2; ALPPL; PALP; PLAP-1; PLAP; |
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Specific References (1) | bs-0220P has been referenced in 1 publications.
[IF=3.864] Xie W? et al. A high-resolution colorimetric immunoassay platform realized by coupling enzymatic multicolor generation with smartphone readout.Analyst. 2018 Jun 11;143(12):2901-2907. other ;
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| CAS | |
| 理論分子量 | ~160 kDa |
| 檢測分子量 | |
| 保存條件 | Stored at 2-8℃. Do not freeze. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品介紹 |
Alkaline phosphatase is highly purified from calf intestine. Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease. Typical uses in the lab for alkaline phosphatases include removing phosphate monoesters to prevent self-ligation, which is undesirable during plasmid DNA cloning. Another important use of alkaline phosphatase is as a label for enzyme immunoassays. CAS：9001-78-9 分子量：dimer ~160 kDa MDL：MFCD00131849 濃度：≥10 mg/mL 活性：≥5500 DEA單位/mg蛋白質 單位定義：一個DEA單位將在37°C的pH 9.8下每分鐘水解1μmol 4-硝基苯磷酸酯（一個甘氨酸單位相當于~3個DEA單位） 性狀：溶液（含5 mM Tris、5 mM MgCl2和0.1 mM ZnCl2的50%甘油溶液，pH 7.0） 用途：堿性磷酸酶可以用來去磷酸酪蛋白和其他蛋白質。堿性磷酸酶也可以用來去磷酸化DNA或RNA的5′端，以防止自連接。用堿性磷酸酶去磷酸化后，DNA或RNA也可以用放射性標記的磷酸鹽（通過T4多核苷酸激酶）標記。 推薦用于抗體和蛋白質結合的高比活性等級。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
