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Contactin 1 Rabbit pAb (bs-13973R)  
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50ul/1180.00元
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產(chǎn)品編號 bs-13973R
英文名稱 Contactin 1 Rabbit pAb
中文名稱 接觸蛋白抗體
別    名 CNTN 1; CNTN; CNTN1; CNTN1_HUMAN; Contactin-1; Contactin1; F3; F3cam; Glycoprotein gp135; gp 135; gp135; Neural cell surface protein F3.  
研究領(lǐng)域 細胞生物  神經(jīng)生物學(xué)  生長因子和激素  細胞粘附分子  糖蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應(yīng) Mouse (predicted: Human,Rat,Pig,Sheep,Cow,Chicken,Dog,Horse)
產(chǎn)品應(yīng)用 Flow-Cyt=3ug/test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 111 kDa
檢測分子量
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Contactin 1: 151-250/1018 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Function:
Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth.

Subcellular Location:
Cell membrane.

Tissue Specificity:
Strongly expressed in brain and in neuroblastoma and retinoblastoma cell lines. Lower levels of expression in lung, pancreas, kidney and skeletal muscle.

DISEASE:
Defects in CNTN1 are the cause of Compton-North congenital myopathy (CNCM) [MIM:612540]. CNCM is a familial lethal form of congenital onset muscle weakness, inherited in an autosomal-recessive fashion and characterized by a secondary loss of beta2-syntrophin and alpha-dystrobrevin from the muscle sarcolemma, central nervous system involvement, and fetal akinesia.

Similarity:
Belongs to the immunoglobulin superfamily. Contactin family.
Contains 4 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

SWISS:
Q12860

Gene ID:
1272

Database links:

Entrez Gene: 1272 Human

Entrez Gene: 12805 Mouse

Entrez Gene: 117258 Rat

Omim: 600016 Human

SwissProt: Q12860 Human

SwissProt: P12960 Mouse

SwissProt: Q63198 Rat

Unigene: 143434 Human

Unigene: 470343 Mouse

Unigene: 4911 Mouse

Unigene: 21397 Rat



產(chǎn)品圖片
Blank control: Mouse kidney. Primary Antibody (green line): Rabbit Anti-Contactin 1 antibody (bs-13973R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: Mouse brain. Primary Antibody (green line): Rabbit Anti-Contactin 1 antibody (bs-13973R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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