| 產(chǎn)品編號(hào) | bs-13997R |
| 英文名稱 | CORO2B Rabbit pAb |
| 中文名稱 | 肌動(dòng)蛋白結(jié)合蛋白2B抗體 |
| 別 名 | Actin binding protein; Clipin-C; CLIPINC; COR2B_HUMAN; CORO2B; Coronin actin binding protein 2B; Coronin; Coronin-2B; Coronin-like protein C; Protein FC96. |
| 研究領(lǐng)域 | 細(xì)胞生物 細(xì)胞類型標(biāo)志物 細(xì)胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Pig,Cow,Chicken,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 55 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CORO2B: 1-100/480 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Coronins are a family of WD repeat-containing, actin-binding proteins that localize to submembraneous areas and regulate cell motility and cytoskeletal rearrangement. Coronin 1A (CORO1A, CLIPINA, CLABP, TACO, p57) can form coiled coil-mediated homotrimeric complexes that influence early phagosome formation. PKC-dependent phosphorylation of Coronin 1B (CORO1B) at Serine 2 regulates leading edge dynamics and cell motility in fibroblasts through interactions with Arp2/3 complex. Coronin 1C (CORO1C, Coronin 3, HCRNN4) is abundant in differentiating Neuro-2a cells, PC-12 cells and primary oligodendrocytes, where it is thought to influence neuron morphogenesis and migration. Coronin 2A (CORO2A, CLIPINB, IR10, WDR2) is a component of the approximately 1.5-2 megadalton N-CoR (nuclear receptor corepressor) complex of 10-12 proteins, which recruits HDACs to generate repressive chromatin. Coronin 7 (CORO7, CRN7) localizes to the Golgi membrane and influences the organization of intracellular membrane compartments and vesicular trafficking. Coronin 2B (CORO2B, CLIPINC) and Coronin 6 (CORO6) are similar to other members of this family, since they possess a conserved basic N-terminal motif and 3-10 WD repeats clustered in one to two core domains. Function: May play a role in the reorganization of neuronal actin structure. Subunit: Binds to F-actin and to vinculin. Subcellular Location: Cytoplasm; cytoskeleton. Tissue Specificity: Expressed predominantly in brain. Similarity: Belongs to the WD repeat coronin family. Contains 5 WD repeats. SWISS: Q9UQ03 Gene ID: 10391 Database links: Entrez Gene: 10391 Human Entrez Gene: 235431 Mouse Entrez Gene: 444835 Xenopus laevis Entrez Gene: 549719 Xenopus tropicalis Omim: 605002 Human SwissProt: Q9UQ03 Human SwissProt: Q8BH44 Mouse SwissProt: Q6DJD8 Xenopus laevis SwissProt: A8WGE3 Xenopus tropicalis Unigene: 551213 Human Unigene: 335229 Mouse Unigene: 24004 Xenopus laevis |
| 產(chǎn)品圖片 |
Tissue/cell: human gliomas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CORO2B Polyclonal Antibody, Unconjugated(bs-13997R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CORO2B Polyclonal Antibody, Unconjugated(bs-13997R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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