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CRNKL1 Rabbit pAb (bs-14065R)  
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產品編號 bs-14065R
英文名稱 CRNKL1 Rabbit pAb
中文名稱 細胞凋亡水解酶樣蛋白1抗體
別    名 1200013P10Rik; 5730590A01Rik; C80326; CRN; Crn crooked neck like 1(Drosophila); HCRN; MGC55327; MGC93283; MSTP021; SYF3; CRNL1_HUMAN; Crooked neck-like protein 1; Crooked neck homolog; hCrn.  
研究領域 細胞生物  發育生物學  細胞周期蛋白  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat (predicted: Sheep,Cow,Chicken,Dog,Horse)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 100 kDa
檢測分子量
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CRNKL1: 201-300/848 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The crooked neck (crn) gene of Drosophila is essential for embryogenesis and is thought to be involved in cell cycle progression and pre-mRNA splicing. A protein encoded by this human locus has been found to localize to pre-mRNA splicing complexes in the nucleus and is necessary for pre-mRNA splicing. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2013]

Function:
The crooked neck (crn) gene of Drosophila is essential for embryogenesis. It is thought to be involved in cell cycle progression and pre mRNA splicing.

Subcellular Location:
Nucleus; nuclear speckle. Cytoplasm. Colocalizes with core spliceosomal snRNP proteins. Also diffusely expressed in cytoplasm.

Tissue Specificity:
Widely expressed. Highly expressed in testis. Not expressed in brain and lung.

Similarity:
Belongs to the crooked-neck family.
Contains 17 HAT repeats.

SWISS:
Q9BZJ0

Gene ID:
51340

Database links:

Entrez Gene: 51340 Human

Entrez Gene: 116481 Rat

Omim: 610952 Human

SwissProt: Q5JY65 Human

SwissProt: Q9BZJ0 Human

SwissProt: P63155 Rat

Unigene: 171342 Human

Unigene: 162694 Rat



產品圖片
Sample: Siha(Human) Cell Lysate at 30 ug HepG2(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug Primary: Anti- CRNKL1 (bs-14065R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100 kD Observed band size: 98 kD
Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CRNKL1 Polyclonal Antibody, Unconjugated(bs-14065R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CRNKL1 Polyclonal Antibody, Unconjugated(bs-14065R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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