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FMNL3 Rabbit pAb (bs-16151R)  
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產(chǎn)品編號(hào) bs-16151R
英文名稱 FMNL3 Rabbit pAb
中文名稱 FMNL3蛋白抗體
別    名 FHOD3; FMNL3_HUMAN; Formin homology 2 domain containing protein 3; Formin like protein 3; WBP3; WW domain binding protein 3.  
Specific References  (2)     |     bs-16151R has been referenced in 2 publications.
[IF=4.966] Pan ZN et al. RAB7 GTPase regulates actin dynamics for DRP1‐mediated mitochondria function and spindle migration in Mouseoocyte meiosis. FASEB J. 2020 Jul;34(7):9615-9627.  IP ;  Mouse.  
[IF=4.966] Pan Z et al. RAB7 GTPase regulates actin dynamics for DRP1‐mediated mitochondria function and spindle migration in Mouseoocyte meiosis. FASEB J . 2020 Jul;34(7):9615-9627.  WB ;  Mouse.  
研究領(lǐng)域 細(xì)胞生物  免疫學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  結(jié)合蛋白  細(xì)胞骨架  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Human (predicted: Mouse,Rat,Cow,Dog)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 117 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human FMNL3: 951-1028/1028 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The protein encoded by this gene contains a formin homology 2 domain and has high sequence identity to the mouse Wbp3 protein. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Function:
FMNL3 is an actin-binding protein that contains a formin homology 2 domain.

Subunit:
Interacts with SRGAP2 (via SH3 domain).

Subcellular Location:
Cytoplasm. Note=Enriched in lamellipodia.

Similarity:
Belongs to the formin homology family.
Contains 1 DAD (diaphanous autoregulatory) domain.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.

SWISS:
Q8IVF7

Gene ID:
91010

Database links:

Entrez Gene: 91010 Human

SwissProt: Q8IVF7 Human

Unigene: 179838 Human



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FMNL3) Polyclonal Antibody, Unconjugated (bs-16151R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control: Molt4. Primary Antibody (green line): Rabbit Anti- FMNL3/FITC Conjugated antibody (bs-16151R-FITC) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-FITC . Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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