| 產品編號 | bs-16619R |
| 英文名稱 | IME1 Rabbit pAb |
| 中文名稱 | 減數分裂誘導蛋白IME1抗體 |
| 別 名 | Meiosis inducing protein; IME1_YEAST; YJR094C; Saccharomyces cerevisiae(strain ATCC 204508/S288c)(Baker's yeast). 釀酒酵母; |
| 研究領域 | 細胞生物 轉錄調節因子 表觀遺傳學 泛素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Rat (predicted: Saccharomyces cerevisiae) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 42 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Saccharomyces cerevisiae IME1: 301-360/360 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Meiosis-inducing protein (IME1) is a master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p. IME1 is required for sporulation and for early sporulation-specific genes expression. It is a positive regulator of SME1/IME2 expression. Function: Transcription factor required for sporulation and for early sporulation-specific genes expression. Positive regulator of SME1/IME2 expression. Directly activates expression of SLZ1 during meiosis. Subunit: Interacts with UME6. Subcellular Location: Nuclear Database links: Entrez Gene: 853556 Saccharomyces cerevisiae SwissProt: P21190 Saccharomyces cerevisiae |
| 產品圖片 |
Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IME1 Polyclonal Antibody, Unconjugated(bs-16619R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
