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KCC2 Rabbit pAb (bs-10295R)  
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產品編號 bs-10295R
英文名稱 KCC2 Rabbit pAb
中文名稱 神經細胞鉀氯離子轉運蛋白抗體
別    名 Electroneutral potassium chloride cotransporter 2; Electroneutral potassium-chloride cotransporter 2; Erythroid K Cl cotransporter 2; Erythroid K-Cl cotransporter 2; Furosemide sensitive K Cl cotransporter; hKCC2; K-Cl cotransporter 2; KCC 2; KCC2; KIAA1176; Neuronal K Cl cotransporter; Neuronal K-Cl cotransporter; S12A5; S12A5_HUMAN; SLC12A5; Solute carrier family 12(potassium chloride transporter) member 5; Solute carrier family 12 member 5.  
研究領域 細胞生物  免疫學  神經生物學  通道蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Rat (predicted: Human,Mouse,Pig,Cow,Chicken,Dog,Horse)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 123 kDa
檢測分子量
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human KCC2: 851-950/1139 <Cytoplasmic>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Sep 2008]

Function:
Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.

Subunit:
Homomultimer and heteromultimer with other K-Cl cotransporters. Interacts with AP2A1.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Brain specific. Detected in neuronal cells.

Similarity:
Belongs to the SLC12A transporter family.

SWISS:
Q9H2X9

Gene ID:
57468

Database links:

Entrez Gene: 57468 Human

Entrez Gene: 57138 Mouse

Entrez Gene: 171373 Rat

Omim: 606726 Human

SwissProt: Q9H2X9 Human

SwissProt: Q91V14 Mouse

SwissProt: Q63633 Rat

Unigene: 21413 Human

Unigene: 252987 Mouse

Unigene: 10513 Rat



產品圖片
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-KCC2 Polyclonal Antibody, Unconjugated(bs-10295R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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