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SPERT Rabbit pAb (bs-17654R)  
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產品編號 bs-17654R
英文名稱 SPERT Rabbit pAb
中文名稱 精子細胞相關蛋白抗體
別    名 CBY2; Chibby homolog 2; FLJ35810; Novel leucine zipper testicular protein; NURIT; Protein chibby homolog 2; Spermatid associated; Spermatid associated protein; Spermatid flower like structure protein; Testis specific leucine zipper protein nurit.  
研究領域 細胞生物  發育生物學  轉運蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Rat (predicted: Mouse,Rabbit,Sheep,Cow,Horse)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 52 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human SPERT: 1-100/448 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 A variety of morphological and molecular changes are required for spermatozoa formation. These steps are temporally guided by the transcription and translation of several testis-specific genes. SPERT (spermatid associated), also known as CBY2 (chibby homolog 2), spermatid flower-like structure protein or NURIT, is a 448 amino acid novel leucine-zipper protein belonging to the chibby family of proteins.Expressed uniquely in the spermatid flower-like structure, SPERT interacts with Nek1, a member of the NIMA-family kinase family that is associated centrosomal stability and ciliogenesis. Containing a leucine-zipper motif and two coiled-coil regions, SPERT is transcribed through the elongation stage of the spermatids. SPERT is absent from mature spermatozoa and is thought to be involved in transporting proteins that are to be discarded via the residual bodies.

Function:
Spermatid-associated protein (SPERT) belongs to the chibby family, SPERT subfamily. It exists as a homodimer and binds to NEK1. There are two named isoforms produced by alternative splicing.

Subcellular Location:
Cytoplasmic membrane bounded vesicle.

Similarity:
Belongs to the chibby family. SPERT subfamily.

SWISS:
Q8NA61

Gene ID:
220082

Database links:

Entrez Gene: 220082 Human

SwissProt: Q8NA61 Human

Unigene: 186363 Human



產品圖片
Sample: Hela(human) Cell Lysate at 40 ug DU145(human) Cell Lysate at 40 ug Primary: Anti-SPERT(bs-17654R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52kD
Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPERT) Polyclonal Antibody, Unconjugated (bs-17654R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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