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RASGRP3 Rabbit pAb (bs-19736R)  
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產品編號 bs-19736R
英文名稱 RASGRP3 Rabbit pAb
中文名稱 Ras蛋白特異鳥嘌呤核苷酸釋放因子3抗體
別    名 Calcium and DAG-regulated guanine nucleotide exchange factor III; Grp3; GRP3_HUMAN; Guanine nucleotide exchange factor for Rap1; Kiaa0846; RAS guanyl releasing protein 3(calcium and DAG regulated); RAS guanyl releasing protein 3 calcium and DAG regualated guanine nucleotide exchange factor iii; Ras guanyl-releasing protein 3; RASGRP3.  
研究領域 細胞生物  信號轉導  G蛋白偶聯受體  G蛋白信號  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 78 kDa
檢測分子量
細胞定位 細胞漿 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human RASGRP3: 601-690/690 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RASGRP3, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008]

Function:
Guanine nucleotide exchange factor (GEF) for Ras and Rap1.

Similarity:
Belongs to the RASGRP family.
Contains 2 EF-hand domains.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Ras-GEF domain.

SWISS:
Q8IV61

Gene ID:
25780

Database links:

Entrez Gene: 25780 Human

Entrez Gene: 240168 Mouse

Omim: 609531 Human

SwissProt: Q8IV61 Human

Unigene: 143674 Human

Unigene: 329203 Mouse



產品圖片
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RASGRP3) Polyclonal Antibody, Unconjugated (bs-19736R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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