| 產品編號 | bs-4373R |
| 英文名稱 | ZNF710 Rabbit pAb |
| 中文名稱 | 鋅指蛋白710抗體 |
| 別 名 | DKFZp547K1113; FLJ00306; FLJ37393; MGC163413; Zinc finger protein 710; ZN710_HUMAN; Znf710. |
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Specific References (1) | bs-4373R has been referenced in 1 publications.
[IF=2.1] Li G et al. Overexpression of antisense long non?coding RNA ZNF710?AS1?202 promotes cell proliferation and inhibits apoptosis of clear cell renal cell carcinoma via regulation of ZNF710 expression. Mol Med Rep. 2020 Jun;21(6):2502-2512. WB ; Human.
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| 研究領域 | 轉錄調節因子 鋅指蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse (predicted: Rat,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 75 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZNF710: 401-500/664 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
ZNF710 (Zinc Finger Protein 710) is a Protein Coding gene. Among its related pathways are Gene Expression. GO annotations related to this gene include nucleic acid binding and transcription cofactor activity. An important paralog of this gene is ZNF366. Function: May be involved in transcriptional regulation. Subcellular Location: Nucleus. Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 11 C2H2-type zinc fingers. SWISS: Q8N1W2 Gene ID: 374655 Database links: Entrez Gene: 374655 Human Entrez Gene: 209225 Mouse GenBank: NM_198526 Human SwissProt: Q8N1W2 Human SwissProt: Q3U288 Mouse Unigene: 459311 Human Unigene: 290925 Mouse |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (ZNF710) Polyclonal Antibody, Unconjugated (bs-4373R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ZNF710) polyclonal Antibody, Unconjugated (bs-4373R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
