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IQSEC3 Rabbit pAb (bs-20333R)  
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產品編號 bs-20333R
英文名稱 IQSEC3 Rabbit pAb
中文名稱 IQSEC3蛋白抗體
別    名 IQ motif and Sec7 domain 3; IQ motif and SEC7 domain-containing protein 3; IQEC3_HUMAN; Iqsec3; KIAA1110; MGC30156.  
研究領域 細胞生物  免疫學  G蛋白信號  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Rat (predicted: Human,Mouse)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 127 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human IQSEC3: 651-750/1185 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 IQSEC3 (IQ motif and SEC7 domain-containing protein 3), also known as KIAA1110, is a 1,182 amino acid protein that belongs to the BRAG family. Localizing to the cytoplasm, IQSEC3 is expressed predominantly in adult brain, where it is observed predominantly in the cerebral cortex and olfactory bulb, with no expression found in the fetal brain. IQSEC3 contains an IQ domain, a PH domain and a SEC7 domain, and interacts with SAP 97 and PSD-95. Acting as a guanine nucleotide exchange factor (GEF) for ARF1, IQSEC3 is encoded by a gene that maps to human chromosome 12p13.33 and mouse chromosome 6 F1. Mutations to the gene encoding IQSEC2 have been linked to non-syndromic X-linked intellectual disability, while both IQSEC1 and IQSEC3 may be candidates for autosomal ID screening. IQSEC3 exists as two alternatively spliced isoforms.

Function:
Acts as a guanine nucleotide exchange factor (GEF) for ARF1.

Subunit:
Interacts with DLG1 and DLG4. {ECO:0000250}.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Expressed specifically in the adult brain, predominantly in the cerebral cortex and the olfactory bulb, but not in the fetal brain. Expressed only in mature neurons, but not in undifferentiated neural stem precursor cells (NSPCs), nor in glioma cells.

Similarity:
Belongs to the BRAG family.
Contains 1 IQ domain.
Contains 1 PH domain.
Contains 1 SEC7 domain.


SWISS:
Q9UPP2

Gene ID:
440073

Database links:

Entrez Gene: 440073 Human

SwissProt: Q9UPP2 Human

Unigene: 536319 Human



產品圖片
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IQSEC3) Polyclonal Antibody, Unconjugated (bs-20333R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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