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CCR-2 Rabbit pAb (bs-23026R)  
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產品編號 bs-23026R
英文名稱 CCR-2 Rabbit pAb
中文名稱 細胞表面趨化因子受體2抗體
別    名 C-C Chemokine receptor 2B; C C chemokine receptor type 2; C C CKR 2; CC chemokine receptor type 2; CC CKR 2; CCCKR2; CCR 2; CCR1L; CCR2A; CCR2B; CCR5L; CD192; CD192 antigen; Chemokine C C motif receptor 2; Chemokine CC Motif Receptor 2; CKR 2; CKR2; CKR2A; CKR2B; CMKBR2; MCP 1 R; MCP1 RECEPTOR; MCP1R; Monocyte chemoattractant protein 1 receptor; Monocyte Chemotactic Protein 1 Receptor; CCR2_MOUSE; C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2; CCR2; Monocyte chemoattractant protein 1 receptor; MCP-1-R.  
研究領域 免疫學  細胞表面分子  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 41 kDa
檢測分子量
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse CCR-2: 301-373/373 <Cytoplasmic>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes two isoforms of a receptor for monocyte chemoattractant protein-1, a chemokine which specifically mediates monocyte chemotaxis. Monocyte chemoattractant protein-1 is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis as well as in the inflammatory response against tumors. The receptors encoded by this gene mediate agonist-dependent calcium mobilization and inhibition of adenylyl cyclase. This gene is located in the chemokine receptor gene cluster region. Two alternatively spliced transcript variants are expressed by the gene. [provided by RefSeq, Mar 2009].

Function:
Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.

Subunit:
Binds to HIV-1 Tat. Interacts with ARRB1.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Post-translational modifications:
N-glycosylated.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
P51683

Gene ID:
12772

Database links:

Entrez Gene: 729230 Human

Entrez Gene: 12772 Mouse

Entrez Gene: 60463 Rat

Omim: 601267 Human

SwissProt: P41597 Human

SwissProt: P51683 Mouse

SwissProt: O55193 Rat

Unigene: 511794 Human

Unigene: 705362 Human

Unigene: 6272 Mouse

Unigene: 211983 Rat



產品圖片
Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CCR-2) Polyclonal Antibody, Unconjugated (bs-23026R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-CCR-2 antibody (bs-23026R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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