| 產(chǎn)品編號 | bsm-33133M |
| 英文名稱 | TAP-Tag Mouse mAb |
| 中文名稱 | TAP-Tag（標簽）單克隆抗體 |
| 別 名 | TAP Tag; TAPTag; Tandem affinity purification |
| 產(chǎn)品類型 | 標簽抗體 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | Mix-mA? |
| 交叉反應 | Recombinant protein |
| 產(chǎn)品應用 | WB=1:1000-5000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant TAP-Tag |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Tandem affinity purification (TAP) is a purification technique for studying protein–protein interactions. It involves creating a fusion protein with a designed piece, the TAP tag, on the end. The original TAP method involves the fusion of the TAP tag to the C-terminus of the protein under study. The TAP tag consists of calmodulin binding peptide (CBP) from the N-terminal, followed by tobacco etch virus protease (TEV protease) cleavage site and Protein A, which binds tightly to IgG. The relative order of the modules of the tag is important because Protein A needs to be at the extreme end of the fusion protein so that the entire complex can be retrieved using an IgG matrix. |
| 產(chǎn)品圖片 |
Sample:
Lane 1: TAP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 2ug
Lane 2: TAP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4ug
Primary: Anti-TAP-Tag (bsm-33133M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
