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CD1C Rabbit pAb (bs-23496R)  
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產品編號 bs-23496R
英文名稱 CD1C Rabbit pAb
中文名稱 T細胞表面糖蛋白CD1C抗體
別    名 CD1c antigen; CD1C antigen c polypeptide; CD1c molecule; CD1C_HUMAN; Cortical thymocyte antigen CD1C; Differentiation antigen CD1 alpha 3 antibody R7; T cell surface glycoprotein CD1c; T-cell surface glycoprotein CD1c.  
Specific References  (2)     |     bs-23496R has been referenced in 2 publications.
[IF=3.412] Xiangfei Meng. et al. Dermal Microvascular Units in Domestic Pigs (Sus scrofa domestica): Role as Transdermal Passive Immune Channels. FRONT VET SCI. 2022; 9: 891286  IHC ;  Pig.  
[IF=3.412] Xiangfei Meng. et al. Dermal Microvascular Units in Domestic Pigs (Sus scrofa domestica): Role as Transdermal Passive Immune Channels. FRONT VET SCI. 2022; 9: 891286  IHC ;  Pig.  
研究領域 免疫學  t-淋巴細胞  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human
產品應用 WB=1:500-2000,Flow-Cyt=1ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 36 kDa
檢測分子量
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD1C: 201-300/333 <Extracellular>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene is broadly distributed throughout the endocytic system via a tyrosine-based motif in the cytoplasmic tail. Alternatively spliced transcript variants of this gene have been observed, but their full-length nature is not known. [provided by RefSeq, Jul 2008]

Function:
Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.

Subcellular Location:
Cell membrane. Endosome membrane. Subject to intracellular trafficking between the cell membrane and endosomes.

Tissue Specificity:
Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues.

Similarity:
Contains 1 Ig-like (immunoglobulin-like) domain.

Gene ID:
911

Database links:

Entrez Gene: 911 Human

Omim: 188340 Human

SwissProt: P29017 Human

Unigene: 132448 Human



產品圖片
Sample: Jurkat(Human) Cell Lysate at 30 ug Hela(Human) Cell Lysate at 30 ug Primary: Anti-CD1C? (bs-23496R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 61 kD
Blank control (Black line): Molt-4 (Black). Primary Antibody (green line): Rabbit Anti-CD1C antibody (bs-23496R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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