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ST2 Rabbit pAb (bs-23758R)  
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產品編號 bs-23758R
英文名稱 ST2 Rabbit pAb
中文名稱 白細胞介素1受體相關蛋白抗體
別    名 DER-4; DER4; FIT 1; Growth stimulation expressed gene 2; homolog of mouse growth stimulation-expressed; Il1rl1; IL33R; ILRL1_HUMAN; Interleukin 1 receptor like 1; interleukin 1 receptor related protein; Interleukin-1 receptor-like 1; Ly84; Lymphocyte antigen 84; Protein ST2; ST2; ST2L; ST2V; Ste2; Suppressor of tumorigenicity 2; T1; T1 protein.  生長刺激表達基因2
Specific References  (1)     |     bs-23758R has been referenced in 1 publications.
[IF=3.263] Li L et al. Sacubitril/valsartan attenuates atrial electrical and structural remodelling in a rabbit model of atrial fibrillation. Eur J Pharmacol . 2020 Aug 15;881:173120.  IHC,WB ;  rabbit.  
研究領域 心血管  細胞生物  免疫學  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 61 kDa
檢測分子量
細胞定位 細胞膜 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ST2 (isoform A,B,C): 1-100/556 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 ST2 is a member of a superfamily containing the Interleukin 1 (IL1) receptor and the Toll-like receptors (TLRs). The TLRs are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. ST2 was originally identified as a protein whose production was stimulated by various proliferation-inducing agents such as PDGF and FGF. More recently, it has been shown to negatively regulate IL1 receptor and Toll-like receptor 4 (TLR4) signaling and to maintain endotoxin tolerance. It has been suggested that the inhibition of TLR4 signaling occurs through the association and sequestering of TLR adaptor molecules such as MyD88 and TIRAP.

Function:
Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function.

Subunit:
Interacts with MYD88, IRAK1, IRAK4, and TRAF6.

Subcellular Location:
Isoform C: Cell membrane.
Isoform B: Secreted.
Cell membrane; Single-pass type I membrane protein.

Tissue Specificity:
Highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. Isoform A is prevalently expressed in the lung, testis, placenta, stomach and colon. Isoform B is more abundant in the brain, kidney and the liver. Isoform C is not detected in brain, heart, liver, kidney and skeletal muscle.

Similarity:
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain.

SWISS:
Q01638

Gene ID:
9173

Database links:

Entrez Gene: 9173 Human

Entrez Gene: 17082 Mouse

Omim: 601203 Human

SwissProt: Q01638 Human

SwissProt: P14719 Mouse

Unigene: 66 Human

Unigene: 289824 Mouse



產品圖片
Sample: 293T(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug A673(Human) Cell Lysate at 30 ug MKN45(Human) Cell Lysate at 30 ug Primary: Anti-ST2 ? (bs-23758R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kD Observed band size: 61 kD
Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti- ST2 (bs-23758R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kD Observed band size: 61 kD
Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ST2) Polyclonal Antibody, Unconjugated (bs-23758R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ST2) Polyclonal Antibody, Unconjugated (bs-23758R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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