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CD47 Rabbit pAb (bs-21460R)  
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產品編號 bs-21460R
英文名稱 CD47 Rabbit pAb
中文名稱 整合素相關蛋白CD47
別    名 Antigenic surface determinant protein OA3; CD 47; CD47 antigen; CD47 glycoprotein; CD47 molecule; CD47_HUMAN; IAP; Integrin Associated Protein; Integrin associated signal transducer; Integrin-associated protein; Leukocyte surface antigen CD47; MER 6; MER6; OA 3; OA3; Protein MER6; Rh related antigen.  
Specific References  (2)     |     bs-21460R has been referenced in 2 publications.
[IF=10.317]   WB ;  human.  
[IF=8.593] Xin Shiyong. et al. ScRNA-seq revealed an immunosuppression state and tumor microenvironment heterogeneity related to lymph node metastasis in prostate cancer. EXP HEMATOL ONCOL. 2023 Dec;12(1):1-21  WB,IF,ICC ;  Human.  
研究領域 腫瘤  神經生物學  細胞粘附分子  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human
產品應用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 33 kDa
檢測分子量
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD47: 19-119/323 <Extracellular>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]

Function:
Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.

Subunit:
Interacts with THBS1 and fibrinogen (By similarity). Monomer. Interacts with SIRPA, SIRPG, UBQLN1 and UBQLN2.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.

Similarity:
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

SWISS:
Q08722

Gene ID:
961

Database links:
Entrez Gene: 961 Human

Entrez Gene: 16423 Mouse

Entrez Gene: 29364 Rat

Omim: 601028 Human

SwissProt: Q08722 Human

SwissProt: Q61735 Mouse

SwissProt: P97829 Rat

Unigene: 446414 Human

Unigene: 31752 Mouse

Unigene: 390865 Mouse

Unigene: 7409 Rat



產品圖片
Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug Lane 2: HL60 (Human) Cell Lysate at 30 ug Primary: Anti-CD47 (bs-21460R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 47 kD
Sample: MOLT-4(Human) Cell Lysate at 30 ug Primary: Anti- CD47 (bs-21460R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 50 kD
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