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TBXT Rabbit pAb (bs-21569R)  
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產品編號 bs-21569R
英文名稱 TBXT Rabbit pAb
中文名稱 Brachyury蛋白抗體
別    名 TBXT_HUMAN; T-box transcription factor T; T; TFT; Brachyury protein; Protein T; SAVA;  
研究領域 心血管  發育生物學  干細胞  轉錄調節因子  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Mouse,Rat (predicted: Human)
產品應用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 48 kDa
檢測分子量
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Brachyury 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 he protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]

Function:
Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site.

Subunit:
Monomer.

Subcellular Location:
Nucleus.

DISEASE:
Neural tube defects (NTD) [MIM:182940]: Congenital malformations of the central nervous system and adjacent structures related to defective neural tube closure during the first trimester of pregnancy. Failure of neural tube closure can occur at any level of the embryonic axis. Common NTD forms include anencephaly, myelomeningocele and spina bifida, which result from the failure of fusion in the cranial and spinal region of the neural tube. NTDs have a multifactorial etiology encompassing both genetic and environmental components. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
Chordoma (CHDM) [MIM:215400]: Rare, clinically malignant tumors derived from notochordal remnants. They occur along the length of the spinal axis, predominantly in the sphenooccipital, vertebral and sacrococcygeal regions. They are characterized by slow growth, local destruction of bone, extension into adjacent soft tissues and rarely, distant metastatic spread. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry. Susceptibility to development of chordomas is due to a T gene duplication.

Similarity:
Contains 1 T-box DNA-binding domain.

SWISS:
O15178

Gene ID:
6862

Database links:

Entrez Gene: 6862 Human

Entrez Gene: 20997 Mouse

Omim: 601397 Human

SwissProt: O15178 Human

SwissProt: P20293 Mouse

Unigene: 389457 Human

Unigene: 913 Mouse



產品圖片
25 ug total protein per lane of various lysates (see on figure) probed with Brachyury polyclonal antibody, unconjugated (bs-21569R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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