| 產品編號 | bs-21897R |
| 英文名稱 | SPRED2 Rabbit pAb |
| 中文名稱 | SPRED2蛋白抗體 |
| 別 名 | C79158; FLJ21897; FLJ31917; SPRE2_HUMAN; MGC163164; Spred 2; Sprouty protein with EVH 1 domain 2 related sequence; sprouty related EVH1 domain containing 2; Sprouty related protein with EVH 1 domain 2; zgc:77284. |
| 研究領域 | 細胞生物 信號轉導 激酶和磷酸酶 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse (predicted: Human,Rat) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 47 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SPRED2 : 381-480/674 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).[supplied by OMIM, Mar 2008] Function: Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. Subunit: Interacts with Ras (By similarity). Homodimer and heterodimer. Able to interact with SPRED1 to form heterodimers. Subcellular Location: Cell Membrane - peripheral membrane protein and Cytoplasmic. Tissue Specificity: Expressed in liver, skin, small intestine, salivary gland and prostate. Post-translational modifications: Phosphorylated on tyrosine. Phosphorylation of Tyr-228 and Tyr-231 are required for ubiquitination. Ubiquitinated; leading to degradation by the proteasome. Similarity: Contains 1 KBD domain. Contains 1 SPR (sprouty) domain. Contains 1 WH1 domain. SWISS: Q7Z698 Gene ID: 200734 Database links: Entrez Gene: 200734 Human Entrez Gene: 114716 Mouse Omim: 609292 Human SwissProt: Q7Z698 Human SwissProt: Q924S7 Mouse Unigene: 59332 Human |
| 產品圖片 |
Sample:
Small inteatine (Mouse) Lysate at 40 ug
Primary: Anti- SPRED2 (bs-21897R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPRED2) Polyclonal Antibody, Unconjugated (bs-21897R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
