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MAP4 Rabbit pAb (bs-21935R)  
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產品編號 bs-21935R
英文名稱 MAP4 Rabbit pAb
中文名稱 微管相關蛋白4抗體
別    名 DKFZp779A1753; MAP-4; MAP4; MAP4_HUMAN; MGC8617; Microtubule associated protein 4; Microtubule-associated protein 4; OTTHUMP00000210723; OTTHUMP00000210725; OTTHUMP00000210727; OTTHUMP00000210730.  
研究領域 細胞生物  信號轉導  細胞周期蛋白  細胞骨架  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Rat (predicted: Mouse,Pig,Sheep,Cow,Dog,Horse)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 121 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human MAP4: 891-990/1156 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The MAP4 (Microtubule associated protein 4)is a non neuronal microtubule associated protein. It promotes microtubule assembly.The phosphorylation of the Pro rich region in the C-terminus negatively regulates MAP4 activity to promote microtubule assembly.

Function:
Non-neuronal microtubule-associated protein. Promotes microtubule assembly.

Subunit:
Interacts with SEPT2; this interaction impedes tubulin-binding.

Subcellular Location:
Cytoplasm, cytoskeleton.

Post-translational modifications:
Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Phosphorylation on Ser-787 negatively regulates MAP4 activity to promote microtubule assembly. Isoform 3 is phosphorylated on Ser-337 and Ser-338.

Similarity:
Contains 4 Tau/MAP repeats.

SWISS:
P27816

Gene ID:
4134

Database links:
Entrez Gene: 4134 Human

Entrez Gene: 17758 Mouse

Entrez Gene: 367171 Rat

Omim: 157132 Human

SwissProt: P27816 Human

SwissProt: P27546 Mouse

SwissProt: Q5M7W5 Rat

Unigene: 517949 Human

Unigene: 217318 Mouse

Unigene: 443428 Mouse

Unigene: 484451 Mouse

Unigene: 203122 Rat



產品圖片
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP4) Polyclonal Antibody, Unconjugated (bs-21935R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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